{1-95479} {Product size range 276-300...} {forward primer 53->74 } CAGATCAAGATGAGCCTGTACG {Tm = 59.9} {PCR product length: 284, GC = 51%} {2-95482} {Product size range 276-300...} {forward primer 75->94 } GTCTAGCATGGGCCACTGAT {Tm = 60.1} {PCR product length: 295, GC = 46%} {3-95491} {Product size range 301-325...} {forward primer 54->73 } TGTTGAGTATCGGTGCTTCG {Tm = 59.9} {PCR product length: 302, GC = 52%} {4-95496} {Product size range 100-125...} {forward primer 98->117} GCGGATCTTGAAGGAATTGA {Tm = 60.2} {PCR product length: 105, GC = 50%} {5-95498} {Product size range 100-125...} {forward primer 105->125} TGATCGTCGTATCACAGCTTG {Tm = 59.9} {PCR product length: 115, GC = 49%} {6-95500} {Product size range 301-325...} {forward primer 79->98 } CAGTTGTGATCCGCAAGAGA {Tm = 60.0} {PCR product length: 305, GC = 50%} {7-95501} {Product size range 151-200...} {forward primer 102->122} GTGGATCATTGAAGAAGCTCG {Tm = 59.8} {PCR product length: 160, GC = 52%} {8-95504} {Product size range 126-150...} {forward primer 128->147} GCATCTCCTCCTCATGAAGC {Tm = 59.9} {PCR product length: 150, GC = 45%} {9-95505} {Product size range 100-125...} {forward primer 110->129} ACCATCGACAACGTTAAGGC {Tm = 60.0} {PCR product length: 101, GC = 55%} {10-95506} {Product size range 126-150...} {forward primer 100->119} CACCAAAGGGTGTTGTGTTG {Tm = 59.9} {PCR product length: 128, GC = 42%} {11-95508} {Product size range 226-250...} {forward primer 184->203} CCGTCTGAAGATTCCATCGT {Tm = 60.1} {PCR product length: 244, GC = 48%} {12-95512} {Product size range 276-300...} {forward primer 50->69 } GCCTCAACAATGGCTCTCTC {Tm = 60.0} {PCR product length: 284, GC = 58%} {13-95514} {Product size range 301-325...} {forward primer 79->99 } GGAGTATGTCAGGCTCAGTGC {Tm = 59.9} {PCR product length: 312, GC = 51%} {14-95515} {Product size range 126-150...} {forward primer 93->110} GCCACATGACCGTTCCTC {Tm = 60.1} {PCR product length: 147, GC = 48%} {15-95522} {Product size range 151-200...} {forward primer 59->78 } GGTTTCGTCGTCTGATGTCA {Tm = 59.7} {PCR product length: 153, GC = 52%} {16-95524} {Product size range 151-200...} {forward primer 98->115} GCCGCATCAAATGTCCTC {Tm = 60.2} {PCR product length: 157, GC = 60%} {17-95537} {Product size range 100-125...} {forward primer 56->75 } GCCTCAACAATGGCTCTCTC {Tm = 60.0} {PCR product length: 119, GC = 59%} {18-95538} {Product size range 226-250...} {forward primer 70->91 } ACCAATGGAGAAGATCAGAAGC {Tm = 59.7} {PCR product length: 233, GC = 43%} {19-95539} {Product size range 226-250...} {forward primer 87->105} TTGGAGCCAATGTTGCACT {Tm = 60.3} {PCR product length: 235, GC = 47%} {20-95542} {Product size range 126-150...} {forward primer 223->241} TCTTCAGTGAGACCGGTGC {Tm = 60.0} {PCR product length: 138, GC = 51%} {21-95550} {Product size range 301-325...} {forward primer 68->89 } AAACCCTAGACTACCTCCTCCG {Tm = 60.0} {PCR product length: 305, GC = 50%} {22-95557} {Product size range 100-125...} {forward primer 113->132} TTGCTCAACAATCCAGGTCA {Tm = 60.2} {PCR product length: 123, GC = 43%} {23-95558} {Product size range 201-225...} {forward primer 64->85 } AGAAATACGAGACCAATCCTGC {Tm = 59.6} {PCR product length: 202, GC = 46%} {24-95564} {Product size range 251-275...} {forward primer 79->96 } AGCCGACGTCTTCGTGAC {Tm = 60.0} {PCR product length: 273, GC = 46%} {25-95566} {Product size range 126-150...} {forward primer 60->79 } ACCTGGTAGCAGAAGCTGGA {Tm = 60.0} {PCR product length: 133, GC = 43%} {26-95569} {Product size range 276-300...} {forward primer 102->122} GTTTCTTGGTGAAGCAGTTCG {Tm = 59.9} {PCR product length: 280, GC = 49%} {27-95571} {Product size range 126-150...} {forward primer 88->107} TCGTTGTTCAAGCACCAGAG {Tm = 60.0} {PCR product length: 136, GC = 47%} {28-95572} {Product size range 226-250...} {forward primer 74->94 } GAGTATCGTGTGTAGGCTCGC {Tm = 59.9} {PCR product length: 245, GC = 47%} {29-95573} {Product size range 151-200...} {forward primer 158->177} GATGTCAACGAGTTGCCTGA {Tm = 59.8} {PCR product length: 159, GC = 53%} {30-95575} {Product size range 151-200...} {forward primer 72->89 } TACGAGGTGGCTCAAGGC {Tm = 59.9} {PCR product length: 170, GC = 56%} {31-95584} {Product size range 151-200...} {forward primer 131->150} TCTCCCTCAAGCAGTCTGGT {Tm = 60.0} {PCR product length: 191, GC = 40%} {32-95586} {Product size range 126-150...} {forward primer 72->91 } TGGAACTTGGGAGTTCGATC {Tm = 60.0} {PCR product length: 148, GC = 49%} {33-95591} {Product size range 100-125...} {forward primer 193->212} CAATGTCCCATGGTGGTACA {Tm = 60.1} {PCR product length: 107, GC = 48%} {34-95594} {Product size range 126-150...} {forward primer 171->190} CTCCTCAACGCAGAACATCA {Tm = 60.0} {PCR product length: 128, GC = 48%} {35-95598} {Product size range 226-250...} {forward primer 141->160} AGACCCGACCCGAACTATCT {Tm = 60.0} {PCR product length: 238, GC = 53%} {36-95607} {Product size range 126-150...} {forward primer 165->185} TCAAGTGTTGTCGTGAGGTTG {Tm = 59.8} {PCR product length: 127, GC = 44%} {37-95611} {Product size range 126-150...} {forward primer 51->72 } ATTGGTGTATTGAAGAGGTGCA {Tm = 59.5} {PCR product length: 149, GC = 41%} {38-95617} {Product size range 201-225...} {forward primer 54->73 } CACCGCTACATTCCTCCAGT {Tm = 60.1} {PCR product length: 223, GC = 57%} {39-95621} {Product size range 100-125...} {forward primer 51->70 } GTTGTTCGCTCCTCAATGGT {Tm = 60.1} {PCR product length: 119, GC = 47%} {40-95643} {Product size range 276-300...} {forward primer 52->71 } GGTCTCTCAGCTAGTCGCCA {Tm = 60.7} {PCR product length: 277, GC = 45%} {41-95645} {Product size range 251-275...} {forward primer 76->95 } ATGACGATTTCTACAGCGGC {Tm = 60.2} {PCR product length: 274, GC = 46%} {42-95646} {Product size range 276-300...} {forward primer 111->128} GTTTCACCCAACCGCAAC {Tm = 59.9} {PCR product length: 284, GC = 52%} {43-95654} {Product size range 226-250...} {forward primer 62->82 } CAATCTTTGTCGTCTCTTCCG {Tm = 59.9} {PCR product length: 229, GC = 45%} {44-95655} {Product size range 151-200...} {forward primer 68->88 } GAGATGGCAGGGTACAGAGTG {Tm = 59.7} {PCR product length: 152, GC = 42%} {45-95659} {Product size range 201-225...} {forward primer 64->83 } CAGCTTGAGGACGGAAGAAC {Tm = 60.0} {PCR product length: 216, GC = 45%} {46-95668} {Product size range 100-125...} {forward primer 108->127} ATCAGACGGCTCTCAATGCT {Tm = 60.0} {PCR product length: 108, GC = 46%} {47-95669} {Product size range 151-200...} {forward primer 66->83 } GTTGCAATGGTGGCCTTC {Tm = 60.1} {PCR product length: 186, GC = 44%} {48-95676} {Product size range 126-150...} {forward primer 74->93 } TCTTGCTCGCCATATCCTTC {Tm = 60.3} {PCR product length: 143, GC = 52%} {49-95679} {Product size range 151-200...} {forward primer 79->98 } CTTCTCGCGGAAGTTCTCAC {Tm = 60.1} {PCR product length: 182, GC = 55%} {50-95685} {Product size range 226-250...} {forward primer 54->71 } TCGACATGACCATCGACG {Tm = 60.2} {PCR product length: 247, GC = 55%} {51-95688} {Product size range 100-125...} {forward primer 145->164} CACAGCGATTCTTGAAGCTG {Tm = 59.7} {PCR product length: 117, GC = 42%} {52-95689} {Product size range 126-150...} {forward primer 254->271} CTGATTCGCCACCTCCAC {Tm = 60.2} {PCR product length: 128, GC = 49%} {53-95693} {Product size range 226-250...} {forward primer 67->86 } CTCTTGGTCGGAGTCGTCTC {Tm = 60.0} {PCR product length: 227, GC = 48%} {54-95694} {Product size range 201-225...} {forward primer 67->88 } TCACTTCAGGATATCATCACCG {Tm = 60.0} {PCR product length: 205, GC = 50%} {55-95701} {Product size range 100-125...} {forward primer 178->199} AGAGTCAGGTGTTGTATGGCTG {Tm = 59.3} {PCR product length: 100, GC = 44%} {56-95702} {Product size range 126-150...} {forward primer 56->75 } ACAATCATGAAGGCCACCAT {Tm = 60.2} {PCR product length: 149, GC = 53%} {57-95706} {Product size range 251-275...} {forward primer 56->74 } GGACCTAAACCGGAGCATG {Tm = 60.5} {PCR product length: 273, GC = 54%} {58-95708} {Product size range 126-150...} {forward primer 50->69 } CTGTGTGCTTCCTGCGACTA {Tm = 60.2} {PCR product length: 129, GC = 44%} {59-95712} {Product size range 100-125...} {forward primer 227->245} TTACGTCGCCTTCACAAGC {Tm = 60.0} {PCR product length: 116, GC = 52%} {60-95713} {Product size range 251-275...} {forward primer 55->74 } CCCGACCACAACGAGAGTAT {Tm = 60.0} {PCR product length: 253, GC = 57%} {61-95714} {Product size range 226-250...} {forward primer 80->100} CAACAAGGAAGGTGTCATTGC {Tm = 60.5} {PCR product length: 250, GC = 41%} {62-95718} {Product size range 226-250...} {forward primer 62->83 } TCTCTATTCAATCTCACACGCG {Tm = 60.4} {PCR product length: 232, GC = 52%} {63-95726} {Product size range 100-125...} {forward primer 146->165} TGCTCAAAGGACACTTCACG {Tm = 60.0} {PCR product length: 101, GC = 44%} {64-95727} {Product size range 226-250...} {forward primer 68->85 } AATCAATCTCCGGCGGAT {Tm = 60.4} {PCR product length: 248, GC = 53%} {65-95729} {Product size range 201-225...} {forward primer 151->170} GGCAGTCATATGTCTCCGGT {Tm = 60.0} {PCR product length: 213, GC = 46%} {66-95733} {Product size range 301-325...} {forward primer 82->101} GATTCATGGCAAGACTCCGT {Tm = 60.1} {PCR product length: 309, GC = 47%} {67-95748} {Product size range 251-275...} {forward primer 63->84 } GAGTATCAAATCAATCTCCGGC {Tm = 59.9} {PCR product length: 272, GC = 54%} {68-95755} {Product size range 276-300...} {forward primer 99->120} ATGAGATCATTACAATCGCTGC {Tm = 59.2} {PCR product length: 282, GC = 45%} {69-95769} {Product size range 100-125...} {forward primer 73->92 } TCAACCCTAAAGCGTATCCG {Tm = 60.1} {PCR product length: 120, GC = 43%} {70-95772} {Product size range 226-250...} {forward primer 52->72 } AGAGAGTCAGAGACGACGACG {Tm = 59.8} {PCR product length: 231, GC = 41%} {71-95781} {Product size range 100-125...} {forward primer 171->188} TCCTGGAACTCCGACGAC {Tm = 59.7} {PCR product length: 104, GC = 53%} {72-95786} {Product size range 251-275...} {forward primer 70->89 } CAAGCAGCTCGTCAGATCAC {Tm = 59.7} {PCR product length: 255, GC = 55%} {73-95798} {Product size range 126-150...} {forward primer 164->184} GACAAAGAGGGTATTCCTCCG {Tm = 59.9} {PCR product length: 146, GC = 51%} {74-95803} {Product size range 126-150...} {forward primer 131->150} CTTCAATGGCCTGGAACCTA {Tm = 60.1} {PCR product length: 143, GC = 49%} {75-95813} {Product size range 126-150...} {forward primer 85->103} TTTCACGGTGGAGAAGACG {Tm = 59.8} {PCR product length: 143, GC = 54%} {76-95817} {Product size range 100-125...} {forward primer 192->212} TGTAGGTGAGCTTAACGACGC {Tm = 60.5} {PCR product length: 102, GC = 46%} {77-95826} {Product size range 126-150...} {forward primer 65->84 } AATCAAGGTCCGAATGCAAC {Tm = 59.9} {PCR product length: 140, GC = 49%} {78-95839} {Product size range 226-250...} {forward primer 58->77 } TGAAGAACCGTTGCTGACAC {Tm = 59.9} {PCR product length: 247, GC = 47%} {79-95854} {Product size range 226-250...} {forward primer 104->123} GGAGCATTGGTTCACCTCAT {Tm = 59.9} {PCR product length: 227, GC = 46%} {80-95866} {Product size range 100-125...} {forward primer 78->98 } ACGAATCGAGATGAGGCTACA {Tm = 59.9} {PCR product length: 123, GC = 46%} {81-95889} {Product size range 126-150...} {forward primer 80->99 } TTGGAGAATTTCTGGAACCG {Tm = 60.0} {PCR product length: 136, GC = 45%} {82-95891} {Product size range 126-150...} {forward primer 152->171} GCAAGAACCCTGACCAAGTC {Tm = 59.7} {PCR product length: 135, GC = 50%} {83-95895} {Product size range 226-250...} {forward primer 83->102} TCAATGGCGTCATCTAGCAG {Tm = 60.0} {PCR product length: 246, GC = 50%} {84-95896} {Product size range 100-125...} {forward primer 170->189} CATCAAGGAGTCTCTTCGGC {Tm = 60.0} {PCR product length: 118, GC = 49%} {85-95897} {Product size range 126-150...} {forward primer 114->133} ATGAGCCAGAGCGAGAGGTA {Tm = 60.1} {PCR product length: 143, GC = 56%} {86-95907} {Product size range 100-125...} {forward primer 103->121} CAATGCGGTAACCAGATCG {Tm = 60.1} {PCR product length: 119, GC = 55%} {87-95915} {Product size range 251-275...} {forward primer 107->128} TTGTAAGAATTGGTGGTGTTGA {Tm = 58.1} {PCR product length: 253, GC = 43%} {88-95917} {Product size range 126-150...} {forward primer 267->286} CTGGAATGCTTCCTATGCGT {Tm = 60.2} {PCR product length: 140, GC = 42%} {89-95926} {Product size range 100-125...} {forward primer 113->130} ACCAGTTGCGTCTGCCTC {Tm = 60.0} {PCR product length: 106, GC = 49%} {90-95928} {Product size range 100-125...} {forward primer 146->165} GCCGATCTCTTCCTCACTTC {Tm = 59.0} {PCR product length: 100, GC = 45%} {91-95948} {Product size range 100-125...} {forward primer 109->126} AAGGAACCGGACCAGGAG {Tm = 60.0} {PCR product length: 102, GC = 48%} {92-95955} {Product size range 226-250...} {forward primer 118->137} AATCACCAAGAGATGGCGTC {Tm = 60.1} {PCR product length: 230, GC = 48%} {93-95978} {Product size range 126-150...} {forward primer 118->138} GGGTATCTTTGAGGTGAAGGC {Tm = 60.0} {PCR product length: 138, GC = 46%} {94-95988} {Product size range 276-300...} {forward primer 99->118} CCTCAGAACCTTCAAGGTCG {Tm = 59.8} {PCR product length: 278, GC = 45%} {95-95991} {Product size range 251-275...} {forward primer 59->79 } TACAACTCCGTTGACTCCTGC {Tm = 60.3} {PCR product length: 252, GC = 47%} {96-95993} {Product size range 100-125...} {forward primer 56->77 } GAGAGTTCATGCAACCTGACTG {Tm = 59.9} {PCR product length: 125, GC = 52%}