PLATE 1 FORWARD AND REVERSE PRIMERS (20 MERS) 34030 Product size range 301-325 forward primer 94-> 115: CCACTACTTATGGTCAGGCTCC Tm = 60.0 reverse primer 405->384: CTAACGGTTTGAAAACTGAGGC Tm = 60.2 PCR product length: 312, GC = 45% 34023 Product size range 251-275 forward primer 85-> 105: TTCAGGTCTTCACCTACGTGG Tm = 60.2 reverse primer 344->324: ACACACTTGATTGAGAACCCG Tm = 60.0 PCR product length: 260, GC = 41 % 33865 Product size range 201-225 forwardprimer 84->104: CACCGCTATCTAGACGTCAGG Tm = 59.9 reverse primer 290->271: ATCGGTGGTGGC'l'l'l'GATAG Tm = 60.0 PCR product length: 207, GC = 53% 34041 Product size range 151-175 forward primer 119- > 137: ACCTCGCCAATCCGATTAC Tm = 59.9 reverse primer 277->260: CCCATTATCGTTGCCTGC Tm = 60.0 PCR product length: 159, GC = 48% 34169 Product size range 351-400 forward primer 47->68 : TGTCAACAACCCTCCTCTATTG Tm = 59.1 reverse primer 397->377: ACTAAAGGAACTGGGCTTTGC Tm = 59.8 PCR product length: 351, GC = 40% 34110 Product size range 100-150 forward primer 150-> 169: AAGATGGTGCTTCATGGAGG Tm = 60.1 reverse primer 287->268: TCCTGACTCTGCAGTGGATG Tm = 60.0 PCR product length: 138, GC = 47% 34183 Product size range 326-350 forward primer 102->121: GATGTCATCAAATCCCAGGG Tm = 60.1 reverse primer 431->412: AAGCACAACCAATCCTCAGC Tm = 60.3 PCR product length: 330, GC = 46% 33989 Product size range 276-300 forward primer 129-> 149: GTCTAGCTTGGGACCGTAACC Tm = 60.0 reverse primer 428- > 410: ACCTTGTAAGCCGACCCTG Tm = 60.1 PCR product length: 300, GC = 45% 33993 Product size range 226-250 forward primer 114->135: AAGTGGTGAGATTCCTTCATCG Tm = 60.5 reverse primer 355->335: AACAGGTTGAACCCTTGTGTG Tm = 59.9 PCR product length: 242, GC = 37% 33896 Product size range 176-200 forward primer 83->102: GGCAGAAGCGTCTAGTTTGG Tm = 60.0 reverse primer 260->241: GACGATAGAGCCGACTTTGC Tm = 60.0 PCR product length: 178, GC = 49 % 34253 Product size range 351-400 forward primer 89-> 109: CATGGACTAGAGAATCTCGCG Tm = 60.0 reverse primer 456->437: CCCCGATCAGATTGAGTAGC Tm = 59.7 PCR product length: 368, GC = 49% 34399 Product size range 326-350 forward primer 91-> 112: TTGGTCAACCTAAAGGTTTTGC Tm = 60.4 reverse primer 431->410: ATGATTAATAAAGCTTTCCCCG Tm = 58.6 PCR product length: 341, GC = 43% 33936 Product size range 276-300 forward primer 74->95: TTTGGTAAACCTGTGATTGTGG Tm = 59.8 reverse primer 369->348: TCAGACTCAACAAGTGGAAGTG Tm = 58.0 PCR product length: 296, GC = 45% 35005 Product size range 201-225 forward primer 113->130: AATCGGCTGATGTCGCTG Tm = 61.0 reverse primer 315->296: TTCCTCCTTCACCGAAACAG Tm = 60.2 PCR product length: 203, GC = 54% 33876 Product size range 176-200 forward primer 129-> 150: GTGACTCCTGAGGTTCATTTCC Tm = 60.0 reverse primer 321- > 301 : AGAGAGCACACAGCGATTAGC Tm = 59.8 PCR product length: 193, GC = 42% 33895 Product size range 100-150 forward primer 139-> 156: TGGCAGGTAGCTTGGCTC Tm = 60.1 reverse primer 264->247: AAGCTTGTTGGCGGTCTG Tm = 60.0 PCR product length: 126, GC = 48% 34195 Product size range 326-350 forward primer 117-> 136: ACACTCTCCAACTTCCACCG Tm = 60.2 reverse primer 449- > 431: GTGTCCCGTCACTTGAACG Tm = 60.1 PCR product length: 333, GC = 57% 34282 Product size range 151-175 forward primer 128-> 149: TGGCTCTCAACAAGAGTAGCTG Tm = 59.8 reverse primer 283->264: CTTTAACGACGGAGGTACGG Tm = 59.6 PCR product length: 156, GC = 53% 34200 Product size range 351-400 forward primer 52->71: GCAAATTGCGAGAGACATTG Tm = 59.4 reverse primer 407->386: GCAGGGTCAGTATTTGTGACTC Tm = 58.7 PCR product length: 356, GC = 42% 33903 Product size range 301-325 forward primer 84-> 102: CAATGTGTGAGCCCAAACC Tm = 60.0 reverse primer 392- > 373: ATGGGGTACCGAACCTAAGC Tm = 60.2 PCR product length: 309, GC = 47% 33893 Product size range 276-300 forward primer 228->247: ATCTTACTCTGTGCCGGGTG Tm = 60.1 reverse primer 524->506: ATTATTGCCGCGTTTACCC Tm = 59.8 PCR product length: 297, GC = 45% 33997 Product size range 201-225 forward primer 254->273: TTGTGGGACGGAGTTAGACC Tm = 60.0 reverse primer 464- > 445: TTGGTGGGTTCTGACCTTTC Tm = 59.9 PCR product length: 211, GC = 49 % 35189 Product size range 351-400 forward primer 58- > 79: TCATCTTCACAGGAAACAATGG Tm = 60.0 reverse primer 412->394: TTTCAGAACACGACGGAGC Tm = 60.0 PCR product length: 355, GC = 44% 34418 Product size range 326-350 forward primer 118- > 138: GAGTTCTCATCGCTCTTCACG Tm = 60.1 reverse primer 467- > 448: GTTTTGGCTTGTGGCCATAC Tm = 60.4 PCR product length: 350, GC = 43% 33930 Product size range 251-275 forward primer 210->229: TGATTGCTTAAGGGGTCTCG Tm = 60.2 reverse primer 472- > 452: ACCCATTCCCTGAACATATCC Tm =59.9 PCR product length: 263, GC = 44% 33995 Product size range 201-225 forwardprimer 167->186: CAGTAGCTAGCCCAACGGAG Tm = 60.0 reverse primer 386->367: CCTCCAGTCAAGTCCTGAGC Tm = 60.0 PCR product length: 220, GC = 49% 33911 Product size range 151-175 forward primer 102- > 120: GGTCTAGGAGCGGTCGAAG Tm = 60.0 reverse primer 276->258: CACCACTGAATGGACCAGC Tm = 60.1 PCR product length: 175, GC = 54% 34144 Product size range 351-400 forward primer 54->73: ATACCATGCCGGAAACTGAG Tm = 60.0 reverse primer 428->408: TCGGTATCTGAACTTTGCAGC Tm = 60.4 PCR product length: 375, GC = 44% 33812 Product size range 351-400 forward primer 72- > 90 : CCATCGACAATGTGAAGGC Tm = 60.1 reverse primer 436->417: ACCACAGGTAAATGGCGATC Tm = 59.8 PCR product length: 365, GC =50% 34031 Product size range 176-200 forward primer 228->247: ACTCCAACGACTCCAACCAC Tm = 60.0 reverse primer 412- > 393: CGGTTGCTGACTCCTTAAGC Tm = 60.0 PCR product length: 185, GC = 52% 34085 Product size range 100-150 forward primer 196->215: CCTAAGAAGAGCGCACCATC Tm = 60.0 reverse primer 334- > 315: TGTTAACTGCAAGCCATTCG Tm = 59.9 PCR product length: 139, GC = 46% 34512 Product size range 351-400 forward primer 72->93: TAGGGTTCCGTCTCAAATTACG Tm = 60.4 reverse primer 451->434: GGGACGATTGCGATTACG Tm = 60.0 PCR product length: 380, GC = 48% 33985 Product size range 276-300 forward primer 70->89: AAAGGGTGGGTTTAGCTTCG Tm = 60.5 reverse primer 364- > 346: CTGGTAACGATGCAATCCG Tm = 60.1 PCR product length: 295, GC = 45% 33916 Product size range 351-400 forward primer 74->93 : GGTACATGCGTGAGTGCATC Tm = 60.2 reverse primer 464- > 445: GGGTACCGAACCCTAAGCTC Tm = 60.0 PCR product length: 391, GC = 42% 34263 Product size range 351-400 forward primer 78- > 99: AAGATTAGGATCGGAATCAACG Tm = 59.5 reverse primer 437->418: AACAACCTTCTTGGCACCAC Tm = 60.0 PCR product length: 360, GC = 49% 35072 Product size range 351-400 forward primer 51->72: CTCAACCTTCTTCGATTTCTCC Tm = 59.3 reverse primer 415->395: GCATTAAATCTCGCATTCCTG Tm = 59.7 PCR product length: 365, GC = 44 % 33890 Product size range 226-250 forward primer 123- > 142: TAAATGCTTCAACCCTTGGC Tm = 60.1 reverse primer 359- > 340: CTTCGCTTGGCTCATTTCTC Tm = 60.1 PCR product length: 237, GC = 43% 33815 Product size range 176-200 forward primer 124->144: AAATCACAAACCGTGAATTCG Tm = 59.9 reverse primer 304->285: ACACCTTTCGGGAATATCCC Tm = 60.0 PCR product length: 181, GC = 48% 33840 Product size range 100-150 forward primer 113- > 132: TGCGACACTAGTTGAGTGGG Tm = 59.9 reverse primer 220->202: TTGTCCCACCACACAAAGC Tm = 60.6 PCR product length: 108, GC = 43% 34216 Product size range 326-350 forward primer 119-> 140: TCAACAGAAGATGATGTTCGAG Tm = 58.0 reverse primer 449->429: CTTCTCTCTCGCTTCTTTCCC Tm = 59.7 PCR product length: 331, GC = 42% 33805 Product size range 276-300 forward primer 111- > 129: GTGTTGCCATCCCTCAATG Tm = 59.9 reverse primer 404->385: CATTCGTTGCGTACCATGAC Tm = 60.0 PCR product length: 294, GC = 46% 34010 Product size range 201-225 forward primer 103->124: ATCAGTCATTGCATCATCATCC Tm = 59.8 reverse primer 313->294: AATTCCAGATCCTCCGATCC Tm = 60.2 PCR product length: 211, GC = 40% 34315 Product size range 151-175 forward primer 111- > 130: TGGTGGAGAACCTTGCTTTC Tm = 60.2 reverse primer 268->249: GCTAACCAAACTCGAGCACC Tm = 59.9 PCR product length: 158, GC = 41 % 34206 Product size range 351-400 forward primer 67- > 87: ATGAGAAAGTTGTTGGCATCG Tm = 60.1 reverse primer 446->427: TCTCTCCGGTGATTCCTTTC Tm = 59.2 PCR product length: 380, GC = 49% 33836 Product size range 301-325 forward primer 100-> 118: GGAGAGTGTGTTGCCATCG Tm = 60.3 reverse primer 411- > 391: GCCTTGTTGCACTTTATTTCG Tm = 59.8 PCR product length: 312, GC = 45 % 33859 Product size range 251-275 forward primer 79->96: CCGATCAGAAACATCGCC Tm = 60.2 reverse primer 337- > 320: CGGCAGGGTTAACGTGTC Tm = 60.1 PCR product length: 259, GC =55% 34473 Product size range 326-350 forward primer 70- > 90: TCGAGGTGACTTTCTTTGAGC Tm = 59. 6 reverse primer 402->385: GAGGAAAAGGCTGGCCTC Tm = 59.9 PCR product length: 333, GC =51% 34883 Product size range 351-400 forward primer 115-> 135: TATAATGGTGATGGCTGGTGC Tm = 60.7 reverse primer 484->465: CCCCTTAAGGCCCTAAACTG Tm = 60.0 PCR product length: 370, GC = 52 % 33901 Product size range 201-225 forward primer 200->219: TGGGGTTCTACAGAGTTGGG Tm = 60.0 reverse primer 414- > 395: ACGACGAAGCAAATATTCCG Tm = 60.1 PCR product length: 215, GC = 41% 34080 Product size range 151-175 forward primer 102-> 121: TGTGCTTGCTGAGACAGGAG Tm = 60.3 reverse primer 261- > 243: AAATGACACGCCTGTTTGG Tm = 59.6 PCR product length: 160, GC = 49% 34122 Product size range 351-400 forward primer 57->76: TTGGCAAACACTCAACGAAG Tm = 59.9 reverse primer 436- > 418: GGACACTTCCTCTCCAGCG Tm = 61.0 PCR product length: 380, GC = 49% 34009 Product size range 301-325 forward primer 117-> 138: GATGATTGAAGGTGGTGAGATG Tm = 59.4 reverse primer 437- > 417: CCTCTTCTGAACCTTCTTCCG Tm = 60.4 PCR product length: 321, GC =51% 34062 Product size range 251-275 forward primer 97- > 117: TTCTCTGTCTCGCTCTTAGCG Tm = 60.0 reverse primer 371->350: GATAGCACCGAAATTCTTTTGG Tm = 60.0 PCR product length: 275, GC = 41% 33983 Product size range 226-250 forward primer 89-> 107: TGTCTGTTCGCGCTTTCTC Tm = 60.3 reverse primer 337->319: TTGACCGTTGCTGGAGAAG Tm = 60.0 PCR product length: 249, GC = 43% 34021 Product size range 176-200 forward primer 130-> 149: GCCAATGAGCTTCCAAGAAG Tm = 60.0 reverse primer 325->307: TTCCCTCTGACCATCCCTC Tm = 60.0 PCR product length: 196, GC = 42% 34222 Product size range 100-150 forwardprimer 291->308: TTTCTGCTCAGGCGGATC Tm = 60.0 reverse primer 397- > 377: CAATG m GGTAGTCCCATGG Tm = 60.1 PCR product length: 107, GC = 47% 34490 Product size range 326-350 forward primer 74->95: AGACCAGTATGTGTACATGGCG Tm = 59.9 reverse primer 412->393: TGGCTCAAGAAGATGGGATC Tm = 60.2 PCR product length: 339, GC =50% 34032 Product size range 276-300 forward primer 133-> 152: GCTTTCCGATTCATGCAGAG Tm = 60.9 reverse primer 428- > 409: TGATTTCACCAGGAAGGTCC Tm = 59.9 PCR product length: 296, GC = 45 % 34268 Product size range 351-400 forward primer 50->69: AATCTTAGACATGGCGGCTG Tm = 60.2 reverse primer 400->383: GCTCGTGCTTGAGCCTTC Tm = 59.8 PCR product length: 351, GC =50% 34359 Product size range 326-350 forward primer 35->54: GATTGATCCACATGGCTTCC Tm = 60.3 reverse primer 361->340: CGAGAGGTACATTGAGATCAGC Tm = 59.0 PCR product length: 327, GC =55% 33870 Product size range 176-200 forward primer 115-> 136: TGAAGATCAACGCAATAAATGC Tm = 60.1 reverse primer 302->281: CACAAGTTTGGCATTAGAGACG Tm = 59.8 PCR product length: 188, GC = 54% 33852 Product size range 100-150 forward primer 116->135: ACGAATGGAACTCCGTGAAC Tm = 60.0 reverse primer 239->219: TGGTAACTGCGGAATAGTTGG Tm = 60.0 PCR product length: 124, GC =50% 34254 Product size range 326-350 forward primer 91- > 110: TTACGCAACAAGCGTCAAAC Tm = 59.9 reverse primer 417->400: TTTAAACCTCCCCTCGCC Tm = 60.0 PCR product length: 327, GC =51% 33833 Product size range 276-300 forward primer 65->85: GGCAGAGCTAGTTGTTGATGG Tm = 59.9 reverse primer 361->342: TCAAGGACTGCTGCAACAAC Tm = 60.0 PCR product length: 297, GC = 46 % 33886 Product size range 226-250 forward primer 108->125: GCATTGCCATTGGAGGTC Tm = 60.0 reverse primer 355->338: AAGTGAGGTGCCGCAGAC Tm = 60.0 PCR product length: 248, GC = 43% 33990 Product size range 251-275 forward primer 161 > 182: GTTAACGTGATCCGACCTAAGG Tm = 59.9 reverse primer 430-> 409: TGATCCTCTGACAACATCAAGC Tm = 60.3 PCR product length: 270, GC = 40% 33841 Product size range 201-225 forward primer 109->130: TCCACATTCAAGACTCAACAGG Tm = 60.1 reverse primer 327->306: AGCGAGTCTGTAAAAGAAACGG Tm = 59.9 PCR product length: 219, GC = 47% 34087 Product size range 151-175 forward primer 153-> 172: GGCTGAGGAACTCTGGTCAG Tm = 60.0 reverse primer 312 > 293: CTTCCTCTTCCTCCGTTTCC Tm = 60.2 PCR product length: 160, GC = 56% 34208 Product size range 351-400 forward primer 105-> 123: TCTCCGTGATGGTGCTCTC Tm = 59.9 reverse primer 458 > 441 : CGCCCTCTTCTAACGGTG Tm = 59.4 PCR product length: 354, GC = 51% 34091 Product size range 301-325 forwardprimer 101-> 121: AGAAGCAATGGAGGAAGAAGC Tm = 60.0 reverse primer 415- > 395: AAAGCTCAACACCATAGTGCG Tm = 60.3 PCR product length: 315, GC = 49% 34133 Product size range 301-325 forward primer 91- > 110: CCATCAAGAATCCACCATCC Tm = 60.1 reverse primer 395- > 375: ATCTGAACTCCCATGGATTTG Tm = 58.8 PCR product length: 305, GC = 46% 34255 Product size range 301-325 forward primer 120- > 139: TGTTCTGGAGAGTGCCATTG Tm = 59.8 reverse primer 426->406: CTCAGAAAGACGATCACCAGG Tm = 59.9 PCR product length: 307, GC = 43 % 33904 Product size range 151-175 forward primer 135- > 154: CTCTAGACTCATCCCTGCCG Tm = 60.0 reverse primer 308->289: AGACCAAGACGAATTGGGTG Tm = 60.0 PCR product length: 174, GC = 49 % 34100 Product size range 351-400 forward primer 81-> 102: TTACGATAATGGGAACATCAGC Tm = 59.0 reverse primer 438->419: ATCTCAGGGAATGTGCATCC Tm = 59.9 PCR product length: 358, GC = 56% 33889 Product size range 301-325 forward primer 130- > 147: ATTGCGCACGTGATGATG Tm = 60.2 reverse primer 437- > 420: GTTCATCACCGGCACCTC Tm = 60.1 PCR product length: 308, GC =55% 33843 Product size range 251-275 forward primer 118- > 136: TTGC m GTAATCGCGGTC Tm = 59.8 reverse primer 369->349: TCCCCAAACAGATATATCCCC Tm = 59.9 PCR product length: 252, GC = 47% 34079 Product size range 201-225 forward primer 199- > 218: CAGAGAGGTGCGGTTTTAGC Tm = 60.0 reverse primer 401- > 383: TTCAGATTGACTGCCCAGC Tm = 59.9 PCR product length: 203, GC = 42% 33855 Product size range 276-300 forward primer 186- > 206: ATGATGGTAACGATGACGAGG Tm = 59.8 reverse primer 483->462: TTGAAGCTAAAGCTGATGATGC Tm = 59.6 PCR product length: 298, GC = 42% 34047 Product size range 226-250 forward primer 119->140: GTGAAGAAGTTGACGAGTGACG Tm = 60.0 reverse primer 354- > 333: GCATCCTCAATTCCTTTTAACG Tm = 60.0 PCR product length: 236, GC = 41 % 33941 Product size range 176-200 forward primer 156-> 176: AGCTCCTGCAGAGAAGAAACC Tm = 60.1 reverse primer 355->336: TGCCGTTGATGAAACTGTTC Tm = 59.7 PCR product length: 200, GC = 44% 34131 Product size range 100-150 forward primer 106-> 123: GATGACGCCGAGCAACTC Tm = 60.5 reverse primer 207-> 190: CAAGCTGCGTTGTGCTGC Tm = 63.0 PCR product length: 102, GC =51% 34556 Product size range 326-350 forward primer 86- > 106: TCTCCATCCAGTGATGAATCC Tm = 59.9 reverse primer 431->412: TGTCCTTGTGCTGAGAATGC Tm = 60.0 PCR product length: 346, GC = 38 % 34312 Product size range 351-400 forward primer 66->85: CTTCTAGGCAGCCACAGAGC Tm = 60.3 reverse primer 421->403: CAACAATTGCTGCCTCTGC Tm = 60.6 PCR product length: 356, GC =50% 34783 Product size range 326-350 forward primer 87- > 108: TCTGCAACTAATCACTTGTTCG Tm = 58.1 reverse primer 414- > 393: CAAGAACTCGAAAATCAGGACC Tm = 60.1 PCR product length: 328, GC = 42% 33954 Product size range 100-150 forward primer 68- > 87: GGAAGTTCGAGCTGCTTTTG Tm = 60.1 reverse primer 210- > 191: CAGAGAAGAAAGGCCAGTGG Tm = 60.0 PCR product length: 143, GC = 48% 33809 Product size range 326-350 forward primer 141- > 161: GGTTGTTGGATTAGGGAGAGG Tm = 59.8 reverse primer 467- > 446: CAAAGCTTATGGAATCGGTAGC Tm = 60.1 PCR product length: 327, GC = 39 % 33913 Product size range 276-300 forward primer 50->69 : ACCAATCTCAACTTCCGACG Tm = 60.1 reverse primer 334->313: AACTTCCAAGAGTTGCTTGACC Tm = 59.8 PCR product length: 285, GC = 48% 33830 Product size range 226-250 forward primer 132-> 152: GATGGGAGTAGAAGGCACTCC Tm = 60.1 reverse primer 357->336: GTAATGCACCACATCATCAATC Tm = 57.8 PCR product length: 226, GC = 37% 33872 Product size range 176-200 forward primer 91- > 112: TCCCAAGCCTAGCAGTAGTAGC Tm = 60.1 reverse primer 278->257: CATCTGCAGAGTAATCACGAGC Tm = 60.0 PCR product length: 188, GC = 49% 34231 Product size range 301-325 forwardprimer 100->119: GTGCGACTCGGGAAGACTAG Tm = 60.0 reverse primer 401- > 382: ATGGTCAGCCAGATTGAAGG Tm = 60.1 PCR product length: 302, GC = 45% 34022 Product size range 251-275 forward primer 107-> 126: TTCAAGTCCCAGGGATCATC Tm = 59.9 reverse primer 372->353: TTCAGCAATGACCCTGTCAG Tm = 59.8 PCR product length: 266, GC = 42% 33976 Product size range 201-225 forward primer 80->99 : ATCCGTAAACACGGTGAAGG Tm = 59.9 reverse primer 292->272: TCTTCCTGGTAATCTCCCAGC Tm = 60.6 PCR product length: 213, GC = 43% 34323 Product size range 151-175 forward primer 102->121: CATGGTATACTGCTGTGCCG Tm = 60.2 reverse primer 256- > 239: TGCTCCAAACGCTTCCTC Tm = 60.1 PCR product length: 155, GC = 52% 34228 Product size range 351-400 forward primer 63->81 : TCCCAGAAACATGGCTGAG Tm = 59.8 reverse primer 443->424: ACACTGCCTCCACCTTTGAC Tm = 60.2 PCR product length: 381, GC = 44% 34356 Product size range 326-350 forward primer 71->92: CTCTAATCTCCGCAACTATGGC Tm = 60.2 reverse primer 418- > 397: CAGGAGCACAACATATAGCTGC Tm = 59.9 PCR product length: 348, GC = 49% 35042 Product size range 351-400 forward primer 66->86: ATCACTTTCTGATGGGTGGTG Tm = 59.8 reverse primer 416->399: AGAGGCACCAGGCTGCTC Tm = 61.7 PCR product length: 351, GC = 46%