PLATE 2 FORWARD AND REVERSE PRIMERS (20 MERS) 34322 Product size range 301-325 forward primer 109-> 129: AGCTCATGCTTCCCTACACTG Tm = 59.5 reverse primer 423 > 404: TTGCCAGTTTCAGATGCAAC Tm = 59.8 PCR product length: 315, GC = 44% 34151 Product size range 251-275 forward primer 109 >129: GAAGGTTGGGCTAAGAAATGG Tm = 60.0 reverse primer 370 >351: TTCCGACGGGCTAAAACTAC Tm = 59.2 PCR product length: 262, GC = 51% 33877 Product size range 201-225 forward primer 273 >292: AGACCCAAACACACCGTCTC Tm = 60.0 reverse primer 478 >459: TTGCCCCAAAAGCTAAACAC Tm = 60.1 PCR product length: 206, GC =50% 34247 Product size range 151-175 forward primer 68-> 88: CCCCAGAATCTACACAACACC Tm = 59.3 reverse primer 224 >206: CACATTCAACGCCCAATTC Tm = 59.9 PCR product length: 157, GC = 43% 33846 Product size range 351-400 forward primer 57 > 76: GGTTTAGTGGACTTCTCGCG Tm = 59.9 reverse primer 448 >429: CTAGCTACGTGGAGGCATCC Tm = 59.9 PCR product length: 392, GC =55% 34235 Product size range 100-150 forward primer 130 > 151: AAACTCGACGGAGATGACTAGC Tm = 59.9 reverse primer 249 >230: ACAACGACTTTGGCGTTACC Tm = 60.0 PCR product length: 120, GC = 46% 33835 Product size range 326-350 forward primer 139 > 158: ATTCTGAGCCTTTGGTGTGG Tm = 60.1 reverse primer 469 >450: ATGCTGCTGTCACTGTTTCG Tm = 60.1 PCR product length: 331, GC = 47% 34689 Product size range 276-300 forward primer 101 > 118: AGCATTCCAATGGCGATC Tm = 59.6 reverse primer 377 > 359: GTATGGTGGCGAGAACGAC Tm = 59.1 PCR product length: 277, GC = 48% 34170 Product size range 226-250 forward primer 169-> 190: AGTATGGATATGGGTTTCCATG Tm = 57.7 reverse primer 397->377: CCTTTGAAACTTCATGTCGAG Tm = 57.5 PCR product length: 229, GC = 40% 34035 Product size range 176-200 forward primer 111- > 128: CAACCGATCGGAACTTCG Tm = 60.2 reverse primer 303->284: CACACATGTTCGGTGACCTC Tm = 60.0 PCR product length: 193, GC = 52% 33923 Product size range 351-400 forward primer 103->122: GCAGCAGCTGTGTTTGAGAG Tm = 59.9 reverse primer 501- > 482: ACCGTTTGACTTACCCATCG Tm = 59.9 PCR product length: 399, GC = 40% 33902 Product size range 326-350 forward primer 56->77: GACTTGTGGGAGTTGGTTTAGC Tm = 60.0 reverse primer 400->383: TGCGCAACTGTTGGGAAG Tm = 62.0 PCR product length: 345, GC = 39 % 34025 Product size range 276-300 forward primer 64->82 : TGAGAAGCTTCAACGACCG Tm = 60.1 reverse primer 348->328: AATCCGACTGGATCAGAACTG Tm = 59.2 PCR product length: 285, GC = 36% 34082 Product size range 226-250 forward primer 111- > 129: AATCCCATGGAAGCTGGAG Tm = 60.0 reverse primer 360- > 341: CTGCGCCTTGCTTTCTTATC Tm = 60.1 PCR product length: 250, GC = 48% 33862 Product size range 176-200 forward primer 180-> 198: AACTCGGTCCTTCGGTTTG Tm = 60.1 reverse primer 361->342: GCACACATCTCACGCTCATC Tm = 60.4 PCR product length: 182, GC = 45 % 34314 Product size range 100-150 forward primer 78->99: CGGAAGTGGTAGCATTATCTCC Tm = 60.0 reverse primer 210->191: AGCTTCCATGGGAGATTGTG Tm = 60.1 PCR product length: 133, GC = 41% 33820 Product size range 326-350 forward primer 126- > 145: TGGAAGATGGTTTCGTCTCC Tm = 60.0 reverse primer 451->430: CTCAGCCTTCATTGGAGTAACC Tm = 60.1 PCR product length: 326, GC = 40% 34333 Product size range 151-175 forward primer 149- > 168: GGCTAACGCTCAGAGTGGTC Tm = 60.0 reverse primer 322- > 302: TCCGTAATTGTTCCTGCTACG Tm = 60.1 PCR product length: 174, GC = 54% 33884 product size range 351-400 forward primer 150-> 167: TGCGGCTGGACTAGGATC Tm = 59.9 reverse primer 508->487: TCCCTTAGAAATCAGCAGAAGC Tm = 60.0 PCR product length: 359, GC = 54% 34207 Product size range 301-325 forward primer 103- > 122: TCGGAATAGTTATCGTCGGC Tm = 60.1 reverse primer 417->398: TCGGATAAGGCCTCAACAAG Tm = 60.2 PCR product length: 315, GC = 47% 34205 Product size range 251-275 forward primer 125-> 144: ATGTTCGGGATGAGGACAAG Tm = 59.9 reverse primer 398- > 378: TCTTGTCAATGAAATCAGCCC Tm = 60.1 PCR product length: 274, GC = 44% 34300 Product size range 201-225 forward primer 127-> 148: ATGACAACCGATTCTGATGATG Tm = 59.8 reverse primer 345->325: AGCCACAGAAGTGAACACAGC Tm = 60.5 PCR product length: 219, GC = 39 % 33878 Product size range 326-350 forward primer 146->166: CTGCAGAAGGGTGTACAAAGC Tm = 59.9 reverse primer 478->461: CCTTGGTTGCCCACTGAG Tm = 60.2 PCR product length: 333, GC = 45% 33905 Product size range 326-350 forward primer 103->121: TACAGAGTCGCCGGAGATG Tm = 60.0 reverse primer 451- > 430: TTGAATCTTGCATCACATTTCC Tm = 59.9 PCR product length: 349, GC = 52% 34128 Product size range 251-275 forwardprimer 89->108: GGCATCTCCAAAAGAAGCTG Tm = 60.0 reverse primer 355- > 335: GATTGGAAAAGTGAGCTTCCC Tm = 60.1 PCR product length: 267, GC =50% 33875 Product size range 201-225 forward primer 141- > 159: GCTCTCCATCAAGCCTTCC Tm = 59.9 reverse primer 360->341: TCCTCTCTTTGTGAATGGGG Tm = 60.0 PCR product length: 220, GC = 47% 34339 Product size range 151-175 forward primer 176-> 196: GATTCCTGCTAACCTTCCACC Tm = 60.0 reverse primer 337->318: GAGATAGCGAAAAGCGGATG Tm = 59.9 PCR product length: 162, GC = 48% 33844 Product size range 351-400 forward primer 66->85 : CTTCCCTCGACGATGAGTTC Tm = 59.8 reverse primer 420->399: TAAAACACACACGATGACTGTG Tm = 57.2 PCR product length: 355, GC =50% 34142 Product size range 301-325 forward primer 99- > 116: TGGCTTTCCCGATCTCTG Tm = 59.9 reverse primer 401->381: TCCTCGGATTAAACACAAACG Tm = 60.0 PCR product length: 303, GC = 53% 33926 Product size range 176-200 forward primer 71- > 89: AAATGGGAAGCCAACAAGC Tm = 60.1 reverse primer 252->233: ATGAGTCCCTTCACCCACAG Tm = 60.0 PCR product length: 182, GC = 52% 34284 Product size range 100-150 forward primer 230- > 249: TTAGCTGCATGAAGGTGTGG Tm = 59.9 reverse primer 360->341: ACAAGGAACCCACTTGTTGC Tm = 60.0 PCR product length: 131, GC = 46% 33929 Product size range 301-325 forward primer 111- > 129: CCCACAGGACATCATCGTC Tm = 59.9 reverse primer 432->415: AAGCCAGAAAAGGCACCC Tm = 60.2 PCR product length: 322, GC = 49% 34163 Product size range 276-300 forward primer 129- > 148: CGATTCAGCTCTTCTCCGTC Tm = 60.1 reverse primer 410- > 393: TTCCTCCATTGCCTCTGG Tm = 59.7 primers forl & revl to for 4 & rev 4 with EST numbers 16 PCR product length: 282, GC = 48% 34188 Product size range 226-250 forward primer 177-> 197: GGCGTTAGTGATGAGTTCAGC Tm = 59.9 reverse primer 415->397: TGGCAGTCGACTTGTCTCC Tm = 60.0 PCR product length: 239, GC = 40% 33933 Product size range 351-400 forward primer 77- > 96: CCTTCCTCCTCGTCCCTTAC Tm = 60.1 reverse primer 433->415: CCTTGTGCAGCACCGTAAC Tm = 60.3 PCR product length: 357, GC =50% Product size range 326-350 forward primer 128-> 148: GACAAAGAGGGTATTCCTCCG Tm = 59.9 reverse primer 477->458: AAGCCCAGTGAAGTGTGGAC Tm = 60.2 PCR product length: 350, GC =50% U ' 34058 Product size range 226-250 forwardprimer 119->140: CACTAGCTGTGAAACCAAGTGC Tm = 60.0 reverse primer 364->345: TCATTGTGAAGCCACTCGAC Tm = 59.8 PCR product length: 246, GC = 48% 33897 Product size range 176-200 forward primer 152-> 171: GGAGCTCCGTGTCTTTCTTG Tm = 60.0 reverse primer 333->313: CGGGTATCGAGTCTTAGGACC Tm = 60.0 PCR product length: 182, GC = 58% 34185 Product size range 100-150 forward primer 140- > 157: CCTGCTGGTCCCATCAAC Tm = 60.0 reverse primer 285->267: GGTGCGAACACCGTCATAC Tm = 60.0 PCR product length: 146, GC = 46% 35190 Product size range 326-350 forward primer 89-> 106: GCTCTCTCAAGCGCCATC Tm = 59.8 reverse primer 414->393: GGTCAGACTGATCAACAGATCC Tm = 58.6 PCR product length: 326, GC = 52% 34109 Product size range 276-300 forward primer 139-> 158: GGGATATGGAATGGTGGATG Tm = 59.8 reverse primer 425- > 406: CTTCACGGATGGATCTTTCC Tm = 59.5 PCR product length: 287, GC = 48% 34125 Product size range 201-225 forward primer 104-> 121:TTTGCAGAGCCTGGAACC Tm = 59.9 reverse primer 307->289: CAGGAATCGGAGGAAGCTC Tm = 59.9 PCR product length: 204, GC = 53% 34115 Product size range 151-175 forward primer 161- > 182: GACGCAAGTTACAGATTCATCG Tm = 59.8 reverse primer 321->304: CGATTCGCGTCCCATATC Tm = 60.0 PCR product length: 161, GC =50% 34868 Product size range 351-400 forward primer 62->83 : CAACGGAATTCAATATGTTCAG Tm = 57.2 reverse primer422->404: TGCTTGGCAGGAAGTCAAC Tm = 60.0 PCR product length: 361, GC = 45% 34174 Product size range 301-325 forward primer 117- > 136: ATATCGGTATTGCTGTGGCC Tm = 59.8 reverse primer 438->417:TTTGACTTCCAGCTATCAGGTG Tm = 59.4 PCR product length: 322, GC = 44% 34224 Product size range 251-275 forward primer 69->88: AGTTTGCTCACATGGTGCTG Tm = 59.9 reverse primer 321- > 304: CGGGGTAGTTGCCAGATG Tm = 60.1 PCR product length: 253, GC = 48% 33882 Product size range 326-350 forward primer 69->86: GGCCTGATGCTCAACGTC Tm = 60.4 reverse primer 413->394: CCAACTTCTCAAGTCCCTCG Tm = 59.8 PCR product length: 345, GC = 44% 33979 Product size range 351-400 forward primer 87- > 107: ATAAGCGCGAATTTGAGAGTG Tm = 59.5 reverse primer 445->425: CGAAGAGGTTCATGAGGACTG Tm = 59.9 PCR product length: 359, GC = 42% 33944 Product size range 201-225 forward primer 139-> 158: GTCGCTCTCCAAACCTGTTC Tm = 59.9 reverse primer 340->321: TGGCAAGAAGTCTGTGTTCG Tm = 60.0 PCR product length: 202, GC = 54 % 34120 Product size range 151-175 forward primer 138->157: CGGGAGAGCAGTTGTAGAGG Tm = 60.0 reverse primer 290->271: TGATCCCTAACCGAAGCATC Tm = 60.0 PCR product length: 153, GC = 44% 34706 Product size range 351-400 forward primer 55->74: CTTATCCACTGACGACGCAG Tm = 59.5 reverse primer 410- > 391: GCCTTGAAGATCATCCCTTG Tm = 59.6 PCR product length: 356, GC = 47% 34233 Product size range 301-325 forwardprimer 116->137: CATATGCATTTCGATTTGTTGG Tm = 60.2 reverse primer 417->397: TGTCTTCATGAGGAATCGAGC Tm = 60.4 PCR product length: 302, GC = 48% 34198 Product size range 251-275 forward primer 112- > 131: CTCTAATCAAACGGCGCTTC Tm = 60.0 reverse primer 381->361: AGTTGCTTTAGCTCATCAGCG Tm = 59.8 PCR product length: 270, GC = 56% 34096 Product size range 226-250 forward primer 167-> 186: CTTATTGCAGGCGTTTCCTC Tm = 59.8 reverse primer 395- > 376: CTCTGGCTTTCAATTCCTGC Tm = 60.0 PCR product length: 229, GC = 44% 34001 Product size range 176-200 forward primer 82-> 101: CTCGAGCTTTCCACGAAATC Tm = 60.0 reverse primer 264->247: CCGCCATGCTCATAAACC Tm = 60.0 PCR product length: 183, GC = 47% 34299 Product size range 100-150 forward primer 141- > 160: TGAGAATGGAATCCTCTGGG Tm = 60.0 reverse primer 251->232: ACACCTTCATCCTCACCACC Tm = 59.8 PCR product length: 111, GC = 47% 34334 Product size range 326-350 forward primer 58->77: TCGTCAATTCAATCTCGCAG Tm = 59.9 reverse primer 390- > 373: AGAACGAAGCAACCGCAG Tm = 60.1 PCR product length: 333, GC = 48% 34250 Product size range 276-300 forward primer 94- > 111: GCATTGGCTTCATCAGCC Tm = 60.3 reverse primer 380->361: ACCTGCACTTTGAACCCATC Tm = 60.0 PCR product length: 287, GC = 53% 33942 Product size range 351-400 forward primer 108-> 125: TACTGCCTCCGCTTCCAC Tm = 59.9 reverse primer 475->454: ATCCTCCTCGAGAGATGAAGTG Tm = 59.8 PCR product length: 368, GC = 52% 34338 Product size range 326-350 forward primer 75->94 : CCCGGTATTGGTGTGGATAG Tm = 60.1 reverseprimer 402->383: TGCCTTATTGTTTAACGGCC Tm = 60.0 PCR product length: 328, GC = 43% 34036 Product size range 176-200 forward primer 111- > 129: TCTCCGTGCTCGACATCTC Tm = 60.1 reverse primer 290->271: AACAGGGTGTTTATGCTCCG Tm = 60.0 PCR product length: 180, GC = 46% 34252 Product size range 100-150 forward primer 231- > 250: TGGGTTCTTAGATCAACGGC Tm = 60.1 reverse primer 345->326: TTCACAAAGTGCGCAAGATC Tm = 60.0 PCR product length: 115, GC =50% 34631 Product size range 326-350 forward primer 55- > 74: AGTTGGCAAGGAGATCCATG Tm = 60.1 reverse primer 381->363: GACCACGGGTGCTATGATG Tm = 59.9 PCR product length: 327, GC = 40 34089 Product size range 276-300 forward primer 72->90: TTCTTCTCCGGGTCAGGTC Tm = 60.2 reverse primer 363->342: GAAAAAGTTAACCGCAGAAACG Tm = 60.2 PCR product length: 292, GC = 44% 34229 Product size range 226-250 forward primer 121-> 141: GCCAGGAGAGGGTTAGG Tm = 60.1 reverse primer 363->344: CGTCTTGTAAGTGCACCAGC Tm = 59.5 PCR product length: 243, GC = 42% 34143 Product size range 251-275 forward primer 81-> 102: GTATTCTCCGTCGATTGTAGCC Tm = 60.0 reverse primer 335- > 316: TCCTCGTAAGTTTGGATCGG Tm = 60.1 PCR product length: 255, GC = 45% 34103 Product size range 201-225 forward primer 179- > 197: TCTCATCGACGTCTCAGGC Tm = 60.1 reverse primer 385->365: AGATACTGCATTTTCCCACCC Tm = 60.2 PCR product length: 207, GC = 49% 34156 Product size range 151-175 forwardprimer 105->125: CACCATATGCTCACAACGATG Tm = 60.0 reverse primer 259->238: CCATTCCTGTAACTGTAAGGCC Tm = 59.9 PCR product length: 155, GC = 49% 33912 Product size range 351-400 forward primer 53->71 : CCAGGTTCGATGTCAGGTG Tm = 60.1 reverse primer 443->424: CGAAGTTATGCCGTACCGAC Tm = 60.5 PCR product length: 391, GC = 37% 34266 Product size range 301-325 forward primer 110- > 129: ATTGCAGCTTGATTCCAAGG Tm = 60.2 reverse primer 412->392: TGTTCTGAAGAAGATGGCTGC Tm = 60.5 PCR product length: 303, GC = 44% 34330 Product size range 301-325 forward primer 92- > 110: AAGGATGTGGAAGCCGTTC Tm = 60.1 reverse primer 402->382: ATGTGACCACCAGAGATACCG Tm = 59.9 PCR product length: 311, GC =50% Product size range 301-325 forward primer 71->92: AAGAAAGAGATGGATCCAGACG Tm = 59.7 reverse primer 386->365: ATGGAACATCTTGCTGAACTTG Tm = 59.2 PCR product length: 316, GC =51% 34757 Product size range 151-175 forward primer 123-> 142: ATGAAGCAATCTGGAGTCGC Tm = 60.4 reverse primer 282->265: AAGTCCCAAGCCGGAGAC Tm = 60.2 PCR product length: 160, GC = 54% 33821 Product size range 351-400 forward primer 120-> 141: GTTGTGTGTGTCAATGTTGGTG Tm = 59.8 reverse primer 480->461: AGTAATGCCACGTCCCAAAC Tm = 59.9 PCR product length: 361, GC = 47% 34278 Product size range 301-325 forward primer 112-> 129: GGGCATGGGACAATATGC Tm = 59.7 reverse primer 435- > 416: GGTTGCCGCATAAAACAGAG Tm = 60.6 PCR product length: 324, GC = 46% 34321 Product size range 251-275 forward primer 103- > 122: TCTCATTCTTCATTTCCGGC Tm = 60.2 reverse primer 358->339: GAACAACTCTTTTGGCCGAG Tm = 59.9 PCR product length: 256, GC =50% 33813 Product size range 201-225 forward primer 249->268: CCAAGCCGAAGAAGATCAAG Tm = 59.9 reverse primer 449->430: TGGTAAACGTAGGGTGAGCC Tm = 60.0 PCR product length: 201, GC = 47% 34213 Product size range 276-300 forwardprimer 95->114: GGTGACCATTGTGGATCCTC Tm = 60.2 reverse primer 377- > 358: TGGCATAGTAACCTCGGGAC Tm = 60.0 PCR product length: 283, GC = 43% 34135 Product size range 226-250 forward primer 163- > 183: GCTCGTTTGGAAGAACTGATG Tm = 59.9 reverse primer 388- > 367: TTACATACAACGTGGCAAAAGC Tm = 60.1 PCR product length: 226, GC = 45% 34068 Product size range 176-200 forward primer 197->217: CGAAGAGTGGAGTTGGAGTTG Tm = 59.9 reverse primer 383->363: GAGAGATGAGCATAGCCATCG Tm = 60.0 PCR product length: 187, GC = 44% 34326 Product size range 100-150 forward primer 79-> 100: ACTTGGTGAGCTAAAGGTCTCG Tm = 59.9 reverseprimer 208->189: CAAGCCCAAAATCAGAGAGC Tm = 60.0 PCR product length: 130, GC = 40% 33945 Product size range 351-400 forward primer 50->67: TACTCATGCCTCGGCCTC Tm = 59.9 reverse primer 435->416: GCTCTTTCCCAGCATCGTAC Tm = 59.8 PCR product length: 386, GC = 52% 33914 Product size range 326-350 forward primer 137-> 156: ACAACCAGACGGTAAATCGG Tm = 59.9 reverse primer 467->447: CTGAGTGCCTTTGTCAGAAGC Tm = 60.2 PCR product length: 331, GC = 47% 34292 Product size range 100-150 forward primer 96- > 115: GCCTCAACAATGGCTCTCTC Tm = 60.0 reverse primer 213-> 194: GCTTGGCAACAGTCTTCCTC Tm = 60.0 PCR product length: 118, GC = 58% 34789 Product size range 326-350 forward primer 77->98: TGTTGGCAATATTTCAGAAAGC Tm = 59.3 reverse primer 410- > 389: AGCTACGATCACAAAACATTGC Tm = 59.3 PCR product length: 334, GC = 45% 34275 Product size range 276-300 forward primer 66->84: TCGGTGTGGGTATGACGAC Tm = 60.4 reverseprimer 359->340: GGAGCGCCTTTATGATCAAC Tm=59.7 PCR product length: 294, GC = 41 % 34097 Product size range 226-250 forward primer 109-> 127: GGAGCAAACATCGTGCATC Tm = 60.2 reverse primer 356->337: ACTCTGATTGCACCACGATG Tm = 59.7 PCR product length: 248, GC = 46% 34171 Product size range 176-200 forward primer 116->136: ACGAGCCATTCACATGTAACC Tm = 59.9 reverse primer 293->276: TTCCCAAAGCAGCTCCAC Tm = 59.9 PCR product length: 178, GC = 49% 34286 Product size range 301-325 forward primer 104-> 125:TTTCAGTAGCAGTTGCAGAAGC Tm = 59.8 reverse primer 409- > 389: GAGGAACAACCTCTTTGCTTC Tm = 58.0 PCR product length: 306, GC = 42% 34118 Product size range 251-275 forward primer 105- > 126: ATCCTTTAATCAATGGCAATGG Tm = 60.0 reverse primer 376->357: CAAAGTCCACGGGAGTTCTC Tm = 59.7 PCR product length: 272, GC =50% 34113 Product size range 201-225 forward primer 121-> 140:TTTGCTTCCACTTTCCCTTG Tm = 60.2 reverse primer 330- > 310: TTCAATCAAATGAGTCCCAGC Tm = 60.1 PCR product length: 210, GC = 42% 34186 Product size range 151-175 forward primer 244->264: ATCACTGACAATGGAGCAACC Tm = 60.0 reverse primer 395->375: TTACCACCGAGTAAGACACCG Tm = 60.0 PCR product length: 152, GC =55% 33916 Product size range 226-250 forward primer 216->235: GATCCATCGTCGTGTGTCAC Tm = 60.0 reverse primer 464->445: GGGTACCGAACCCTAAGCTC Tm = 60.0 PCR product length: 249, GC = 37% 33866 Product size range 326-350 forward primer 156-> 175: AACACTTCCATCGCCATCTC Tm = 60.1 reverse primer 491->474: TTGACGCAGCTGCAAGAG Tm = 60.0 PCR product length: 336, GC = 51% 34504 Product size range 301-325 forward primer 60- > 80: CTCGATTCCAATCTCTCTCCC Tm = 60.2 reverse primer 367->348: TTGTCAGCGTCAAGACCAAC Tm = 59.9 PCR product length: 308, GC = 45%