PLATE 3 FORWARD AND REVERSE PRIMERS (20 MERS) 34574 Product size range 301-325... forward primer 135- > 155: CAACTGATTTGAGGCTGAACC Tm = 59.7 reverse primer 438- > 419: TGATTGCCTGAAACAGCAAC Tm = 59.8 PCR product length: 304, GC = 39% 34204 Product size range 251-275 forward primer 140- > 159: AGGACCAGTGAACATACCGG Tm = 59.8 reverse primer 411- > 392: GCCTCTTCTGCTGGTCAATC Tm = 60.0 PCR product length: 272, GC = 51% 34088 Product size range 201-225 forward primer 110->127: ATCTTGCCTCCTCTCGGC Tm = 60.5 reverse primer 313->294: AGTCCCAAAGGGAGAAGAGC Tm = 59.8 PCR product length: 204, GC = 45% 34422 Product size range 151-175 forward primer 118-> 139: GACAACAAGmACAACCGTGC Tm = 59.6 reverse primer 285->265: ACTGGGGTTGATTGAACTTCC Tm = 60.2 PCR product length: 168, GC = 43% 34595 Product size range 100-150 forward primer 90- > 109: TTCCGCATATGGATAAAGCC Tm = 59.9 reverse primer 225->206:TTTGGCGTAGGGCGTATAAC Tm = 60.0 PCR product length: 136, GC = 43% 33919 Product size range 326-350 forward primer 50->71: TATCCTTCCTCGAAACAAGAGC Tm = 59.9 reverse primer 388->368: ACAACACCAGAAAGCTGATGG Tm = 60.2 PCR product length: 339, GC = 49% 34605 Product size range 276-300 forward primer - 63- > 84 : AGCTTCCCTGTAACTTCTGTGC Tm = 59.9 reverse primer 339- > 318: GCATGTCACAGTCTTTCACTGG Tm = 60.8 PCR product length: 277, GC = 45% 34140 Product size range 226-250 forward primer 104-> 125: CTTGTCCATTCTCCAAATCTCC Tm = 59.9 reverse primer 333- > 312: CTGGTGGGTACTTAGTGAAGCC Tm = 60.1 PCR product length: 230, GC = 46% 34173 Product size range 176-200 forward primer 143-> 162: ATTCAAATGGCCATGAGGAG Tm = 59.9 reverse primer 322->303: GCTTAGCAGATGGCTGAACC Tm = 60.0 PCR product length: 180, GC =51% 34367 Product size range 351-400 forward primer 56. >77: AGTTCGTCTCTACCTCTGAGGC Tm = 59.2 reverse primer 410->389: AACCAGATCCTTCAACGATAGC Tm = 59.6 PCR product length: 355, GC = 45% 33823 Product size range 326-350 forward primer 58->79: CAGAGATAATGGCGTACAGTGC Tm = 59.8 reverse primer 385- > 366: ACCTGCACTTTGAACCCATC Tm = 60.0 PCR product length: 328, GC = 53% 34386 Product size range 276-300... forward primer 57->77: GACACAACGACTCTCAGGAGC Tm = 60.0 reverse primer 344- > 324: TCAGTCTCTAGACAGCAGCCC Tm = 59.8 PCR product length: 288, GC = 47% 34285 Product size range 226-250 forward primer 90 > 109: ATGGAAGGCATTGAATCTGG Tm = 59.9 reverse primer 319->300: CAAGACGGCCTTGTTTCAAG Tm = 60.8 PCR product length: 230, GC = 47 % 34265 Product size range 176-200 forward primer 88 > 106: TCGCTGAAACATGCTCTCC Tm = 60.1 reverse primer 282->263: GAACCAAATCCCTGTCCATG Tm = 60.2 PCR product length: 195, GC = 46% 34357 Product size range 100-150 forward primer 158-> 178: GCTTGAAACCACAGAATCTCG Tm = 59.9 reverse primer 287->267: TGTGTCATAAGCAAGACGTGC Tm = 59.9 PCR product length: 130, GC = 45% 33920 Product size range 326-350 forward primer 120-> 139: CCGGGATTATATTGGCATTG Tm = 60.0 reverse primer 445->426: CCTCTTCGCTATTACGCCAG Tm = 60.0 PCR product length: 326, GC = 43% 34470 Product size range 151-175 forward primer 51 > 70: CTTGCATAACCCAACCAAGG Tm = 60.4 reverse primer 223->204: TGGATTTAGCGGATTTCCAC Tm = 59.9 PCR product length: 173, GC = 40% 34037 Product size range 351-400 forward primer 73 >92 : TATCCCGTTGTGAGGGAGAG Tm = 60.1 reverse primer 435- > 416: TCATTCATAGCAGGCTGCAG Tm = 60.1 PCR product length: 363, GC = 45% 34360 Product size range 301-325 forward primer 123-> 143: CTGCAATTGGAGTTCTCAAGG Tm = 59.9 reverse primer 425->404: GTGGATAAAGACGAGTTCCAGC Tm = 60.1 PCR product length: 303, GC = 49% 34345 Product size range 251-275 forward primer 75->96: GATAGAGGTGAACGTTTGGAGC Tm = 60.1 reverse primer 343->325: TAAAAAGTCGTGGCAACGC Tm = 59.9 PCR product length: 269, GC = 46% 34273 Product size range 201-225 forward primer 107-> 127: TGCTAAGTATGGTGGTTTCGG Tm = 60.0 reverse primer 319->300: TCCATCAGATTTGGAGGAGG Tm = 60.0 PCR product length: 213, GC = 48% 33931 Product size range 326-350 forward primer 74->92: TTGTGCTGCTGCTGAGTTG Tm = 59.9 reverse primer 415->395: GCATTCTCATACGCGAGTAGC Tm = 60.0 PCR product length: 342, GC = 46% 33992 Product size range 326-350 forward primer 104-> 125: GTTGCATCGGTAACAGTTTCTG Tm = 59.7 reverse primer 445->424: CACTTCTGTTGATTTGCTCAGG Tm = 59.9 PCR product length: 342, GC = 45 % 34243 Product size range 251-275 forwardprimer 97->116: GGATCGTCCATTTGGTATGG Tm = 60.0 reverse primer 351->332: ATGCTTTTCAACAACGACCC Tm = 60.0 PCR product length: 255, GC = 44% 34098 Product size range 201-225 forward primer 103- > 121: TAACGGTGCGATAACGGAG Tm = 59.7 reverse primer 306->288: AATGTCATACGAGCGACGC Tm = 59.8 PCR product length: 204, GC = 57% 34517 Product size range 151-175 forward primer 74->93: TCACTTGGAACACTTGCTGC Tm = 60.0 reverse primer 228->209: ACAACCACCACAAGCTTTCC Tm = 60.0 PCR product length: 155, GC = 46% 34503 Product size range 301-325 forward primer 92- > 112: GTATTGACCCACCATCATTGG Tm = 59.9 reverse primer 416->396: TTCTAATGGTGTCCTTGACCG Tm = 60.0 PCR product length: 325, GC = 45% 34117 Product size range 176-200 forward primer 165- > 182: CATGGGTTCAGGTCTCGG Tm = 60.0 reverse primer 356->338: TCCGATGAGAAGCATCGAC Tm = 59.9 PCR product length: 192, GC = 52% 34353 Product size range 100-150 forward primer 236->253: CCATGCGGTTTATTTGGC Tm = 59.9 reverse primer 375- > 356: ACAAGACACCGAAGAATCCG Tm = 60.1 PCR product length: 140, GC = 45 % 33940 Product size range 326-350 forward primer 142->161: AGCAGAAGGTCTCGAGCTTG Tm = 59.9 reverse primer 470->451: CTCTTCGCTATTACGCCAGG Tm = 60.0 PCR product length: 329, GC = 40% 34531 Product size range 276-300 forward primer 78- > 95: CCGCTGCAAAGAAAGGAG Tm = 59.7 reverse primer 368->349: TCATTCTTCTGCGGACAGTG Tm = 60.0 PCR product length: 291, GC = 47% 34167 Product size range 226-250 forwardprimer 143->161: GGACCAATCAATCCCAAGG Tm = 60.1 reverse primer 390->371: AGGGCAAACACATTGACCTC Tm = 60.0 PCR product length: 248, GC = 44% 34416 Product size range 351-400 forward primer 57- > 77: GCGACTACAGTCTCCATCAGC Tm = 60.0 reverse primer 411- > 392: TAAATCCTAAGGCGGCACTC Tm = 59.3 PCR product length: 355, GC =50% 34261 Product size range 326-350 forward primer 123-> 142: CATGCTATGTGGCTCAATGG Tm = 60.1 reverse primer 456- > 437: CATCAGCATCGATATCACGG Tm = 60.1 PCR product length: 334, GC = 46% 34210 Product size range 226-250 forward primer 143-> 162: TTTTTGGGTTGTGAAGAGCC Tm = 60.1 reverse primer 381->364: GTCCGTACGGTGTTTCCG Tm = 60.0 PCR product length: 239, GC = 43% 34107 Product size range 176-200 forward primer 192- > 209: CATCGCATTCCAAGGGAC Tm = 60.0 reverse primer 387->370: TAGGGGATGAGAAACGCG Tm = 59.7 PCR product length: 196, GC = 43 % 34461 Product size range 100-150 forward primer 85-> 105: TGTTGTTAGCTGCAATCGATG Tm = 59.9 reverse primer 206-> 185: GAGTTCTGGATTGAGCATGTTG Tm = 59.7 PCR product length: 122, GC = 37 % 33925 Product size range 326-350 forward primer 176-> 196: AAGCCTGTCACTGAGTTCTGG Tm = 59.5 reverse primer 524->504: CCCGTAAATGTATGCTATCCG Tm = 59.4 PCR product length: 349, GC = 38% 34561 Product size range 276-300 forward primer 80-> 101: CTGTGTTCTAAATTCCTGGACG Tm = 58.8 reverse primer 364- > 344: GGAAGAGAAGAAACCACCAGG Tm = 60.1 PCR product length: 285, GC = 46% 34094 Product size range 201-225 forward primer 101- > 121: ACCCAACCCGTATCAGTATCC Tm = 60.0 reverse primer 320->299: AAGAACCAGAAGCAGTTTGACC Tm = 59.8 PCR product length: 220, GC = 45% 34370 Product size range 151-175 forward primer 97-> 116: ATTAATCCCGGTGATGAACG Tm = 59.6 reverse primer 262->243: CGCCGAATCTAGCTCCATAG Tm = 60.0 PCR product length: 166, GC = 45% 34040 Product size range 351-400 forward primer 68->87: TCCTTCCGAGTTCTTCATGC Tm = 60.3 reverse primer 439->421: TCCAGCCTCAGCATCTCTG Tm = 60.2 PCR product length: 372, GC = 42% 34396 Product size range 301-325 forward primer 145-> 164: AGACGGCTCTCAATGCTTTC Tm = 59.6 reverse primer 445->427: GGGAGGAATTCTTCTCCCG Tm = 60.9 PCR product length: 301, GC =50% 34597 Product size range 251-275 forward primer 65->84: 'TTTCGATGGCGGAGATTTAC Tm = 60.0 reverse primer 335- > 316: AATGTTGAAGAAGCGGGATG Tm = 60.1 PCR product length: 271, GC = 41 % 34004 Product size range 326-350 forward primer 166->183: TCACCGGAGGCAAGAGAG Tm = 60.1 reverse primer 503->484: AAAACAATTCCACACCGCTC Tm = 60.0 PCR product length: 338, GC = 56% 34448 Product size range 351-400 forward primer 52->73 : CAAACACCACCAATCTTCTCTC Tm = 58.7 reverse primer 410->392: CAAACGCTCGTGATTCGAC Tm = 60.4 PCR product length: 359, GC = 44% 34258 Product size range 201-225 forward primer 226- > 247: CTCTTTCCTTTGCTCAGACATG Tm = 59.1 reverse primer 426- > 409: CGTAGCCGTTGGGTTCAC Tm = 60.1 PCR product length: 201, GC = 47% 34532 Product size range 151-175 forwardprimer 99->119: ATCTATGCATGTCTATGCGGG Tm = 60.0 reverse primer 257->236: CCATAGAGAAACTCCTGGTTGG Tm = 60.0 PCR product length: 159, GC =50% 34026 Product size range 351-400 forwardprimer 98->117: CCTGGTAGCAGAAGCTGGAG Tm = 60.1 reverse primer 495->475: CTTTCCTAACATTGCTGCTGC Tm = 60.0 PCR product length: 398, GC = 45% 34400 Product size range 301-325 forward primer 139- > 158: TCCGGCTTCTTTCTCACTTC Tm = 59.6 reverse primer 447->430: CACCTTTTGCCGCTCAAC Tm = 60.4 PCR product length: 309, GC =51% 34277 Product size range 251-275 forward primer 69- > 90: AACTGATCTTTGCAACATGACG Tm = 60.2 reverse primer 331- > 314: GACGACCACCACCACCTC Tm = 59.9 PCR product length: 263, GC =50% 34200 Product size range 226-250 forward primer 114- > 133: TGGGGTTATGCAGAAGTTCC Tm = 59.9 reverse primer 361- > 341: CTTTAGCCTCTGCAACAAACG Tm = 60.1 PCR product length: 248, GC = 42% 34296 Product size range 176-200 forward primer 157-> 177: TCCAAAAGTTCGACAATTTCG Tm = 60.1 reverse primer 348->329: CTATGTGACAGCACAACCCG Tm = 60.2 PCR product length: 192, GC = 44% 34491 Product size range 100-150 forward primer 90- > 110: TGCTTGTGAAGCAATCAGATG Tm = 60.0 reverse primer 229->210: GAGTTGCTGAAGACGAAGGG Tm = 60.0 PCR product length: 140, GC = 46% 33952 Product size range 326-350 forward primer 93-> 114: GCAAACCCTAAATCAAATCTGG Tm = 59.8 reverse primer 437->416: CTTTGGCTCAGTCCAATTAACC Tm = 60.0 PCR product length: 345, GC = 41% 34351 Product size range 276-300 forward primer 104- > 122: CTCCAGCAACTCTCTTGCG Tm = 59.9 reverse primer 379->359: CTTAACCGGGAAGGTAACTGC Tm = 60.0 PCR product length: 276, GC = 45% 34646 Product size range 351-400 forward primer 49->69: CAAATCCATCTCCCACTCTTC Tm = 58.6 reverse primer 399->378: TGATGTTACCACCAATGAAAGC Tm = 59.9 PCR product length: 351, GC = 52% 33927 Product size range 326-350 forward primer 114-> 135: TAATGATGAGTATGGGAGCTGG Tm = 59.1 reverse primer 439->421: GGCCGCAGATTTAGGTGAC Tm = 60.6 PCR product length: 326, GC = 34% 34106 Product size range 176-200 forward primer 87->106: GGGCTGAGAAGAGCAGACAC Tm = 60.1 reverse primer 273->254: CTTCCGGAAAACTCCTCATG Tm = 59.7 PCR product length: 187, GC = 47% 34447 Product size range 100-150 forward primer 190- > 209: ACCACCACTTCCCAACAGAG Tm = 60.0 reverse primer 316->295: GGGATTGATAGTTACCTGCTGC Tm = 60.0 PCR product length: 127, GC = 42% 33908 Product size range 326-350 forward primer 57- > 76: GAAGTCGGACTCTGATTCGG Tm = 59.8 reverse primer 387->369: GCGTCATCATCCATCCTTG Tm = 60.0 PCR product length: 331, GC = 46% 34368 Product size range 276-300 forward primer 106->125: CCGAGTTCATAGCCACCTTC Tm = 59.7 reverse primer 403->382: CTGAAACCCTTTTGACAACTCC Tm = 60.0 PCR product length: 298, GC = 49 % 34264 Product size range 226-250 forward primer 93- > 111: TCCTCCGATAGATTTCGCC Tm = 60.1 reverse primer318- > 297: CCAAACACTTCAGCTAAAACCC Tm = 60.0 PCR product length: 226, GC = 49% 34246 Product size range 251-275 forward primer 103-> 122: TTGCATCTCGTGGACTTGAC Tm = 59.8 reverse primer 376- > 356: TGAACACCATTTAGAGTGGCC Tm = 60.0 PCR product length: 274, GC = 49% 34293 Product size range 201-225 forward primer 118- > 139: AAACAGAAGACGAGGACTTTGC Tm = 59.9 reverse primer 325- > 306: GTTATGGGATCATCAACCGG Tm = 60.0 PCR product length: 208, GC =51% 34484 Product size range 151-175 forward primer 232- > 249: TGGAGCAGAGGGATGGAG Tm = 59.9 reverse primer 387- > 368: AACTGTGATGAACTTCCCGC Tm = 60.1 PCR product length: 156, GC = 47% 34076 Product size range 351-400 forward primer 74->94: ACGAAACCATGTTCTTGTTCG Tm = 60.0 reverse primer 444->426: AGTTGCAAGGGCTTCATCC Tm = 60.2 PCR product length: 371, GC = 42% 34398 Product size range 301-325 forward primer 94->113: CCTCGAGGAGCAGTTCAATC Tm = 60.0 reverse primer 397- > 378: AAGTACAAGTTCGTTGGCCC Tm = 59.1 PCR product length: 304, GC = 49% 34591 Product size range 301-325 forward primer 50->70: TTCAACCGGAGTAATACACGC Tm = 60.0 reverse primer 364- > 344: TCTTTAGCTAGCCTTGCATCG Tm = 59.8 PCR product length: 315, GC = 44% 34309 Product size range 151-175 forward primer 128->148: ACAAGACCATGGACAGTCTGG Tm = 60.0 reverse primer 290->273: CTGTGCCAGCTGTTGCTG Tm = 60.3 PCR product length: 163, GC = 56% 33996 Product size range 351-400 forward primer 81->98 : CATTATCGGTGCCCTTGC Tm = 60.0 reverse primer 454- > 433: TGATCCGAATATTGTGTTACGC Tm = 59.9 PCR product length: 374, GC = 43% 34411 Product size range 301-325 forward primer106- > 124: GTCACCTCAACGCATCACC Tm = 60.1 reverse primer 407- > 388: CCGAwl l l AAGAGCTGGCCTG Tm = 62. 1 PCR product length: 302, GC = 49% 34274 Product size range 251-275 forward primer 167- > 187: TGACGATATCCAGGGTACTGC Tm = 60.0 reverse primer 419- > 399: GTGCTGAAGAGATTCTTTGCG Tm = 60.1 PCR product length: 253, GC = 51% 34119 Product size range 201-225 forward primer 172->189: CTCGAGATCCGAGCCAAG Tm = 59.6 reverse primer 392- > 375: GTCCTTTGCCACCAGTGG Tm = 60.1 PCR product length: 221, GC = 44% 34402 Product size range 276-300 forward primer 131-> 152:TTTGAAGACTTGATGATCCCTG Tm = 59.2 reverse primer 407- > 390: CACGCCTCTGAAATGGCC Tm = 62.8 PCR product length: 277, GC = 43% 34238 Product size range 226-250 forward primer 148- > 167: TAAATCCTCCGCAAGATTCG Tm = 60.2 reverse primer 391->374: AGGGGCAGATTCAGGACC Tm = 60.0 PCR product length: 244, GC =50% 34257 Product size range 176-200 forward primer 245->264: CACGATCACAATAACGCCAC Tm = 60.0 reverse primer 424->404: GATCGCTCCAGAAACTAACCC Tm = 60.1 PCR product length: 180, GC = 49% 34465 Product size range 100-150 forward primer 36->57: TAAGAAGACGATGGAGATTGGG Tm = 60.4 reverse primer 154- > 133: TTGTAGGATGAGACAATGCTGG Tm = 60.1 PCR product length: 119, GC = 38 % 33987 Product size range 326-350 forward primer 70->90: TCATCATGGCAACTTCTCTCC Tm = 60.2 reverse primer 396->377: CGGTACCCTTTACCTCCATG Tm = 59.3 PCR product length: 327, GC = 54% 34428 Product size range 351-400 forward primer 51->72: ACCATCGTTTCTCTAATCTCCG Tm = 59.6 reverse primer 409->388: CAGGAGCACAACATATAGCTGC Tm = 59.9 PCR product length: 359, GC = 49 % 34015 Product size range 326-350 forward primer 94-> 114: TTGCTCTCTCTCTCTCAACGC Tm = 60.0 reverse primer 426->405: CATCAAGACCCCTTACAGAAGG Tm = 60.0 PCR product length: 333, GC = 46% 34162 Product size range 100-150 forward primer 225->242: ACCCAGCAACGCTAGACG Tm = 60.0 reverse primer 338- > 319: CCTGCTCTGCAATTTCCTTC Tm = 60.0 PCR product length: 114, GC = 47% 34024 Product size range 326-350 forward primer 104-> 122: TGGGATGAAACTGTAGCCG Tm = 59.7 reverse primer 429->412: GCTCAACGGTGCTTGCTC Tm = 60.7 PCR product length: 326, GC = 56% 34350 Product size range 276-300 forward primer 86->105: ATCGGAGGTTTCTCCGAATC Tm = 60.4 reverse primer 383->364: GAACAAATGTACCATCGGCC Tm = 60.2 PCR product length: 298, GC = 41% 34196 Product size range 226-250 forward primer 171- > 191: GAGGCAAAAGGGAGTAATTGC Tm = 60.1 reverse primer 409->392: CACGAGTCGCAATGAACG Tm = 60.0 PCR product length: 239, GC = 49% 34127 Product size range 176-200 forward primer 119-> 140: TCCAATTGTGTTCAATTTCTCG Tm = 60.0 reverse primer 300->279: CCATAACCCTCACTACGTAGGC Tm = 59.9 PCR product length: 182, GC = 43% 34495 Product size range 301-325 forward primer 115->135: GATATGGCTGCTCTTCAGTGC Tm = 60.0 reverse primer 430->412: TCCTGGTCTTCCTTGACCC Tm = 60.0 PCR product length: 316, GC = 49% 34197 Product size range 251-275 forward primer 96- > 114: GATTCTGGCCGAGGAAGAG Tm = 59.9 reverse primer 367->348: CCTTGAGAGCCCAGTTGATC Tm = 59.8 PCR product length: 272, GC = 48 % 34288 Product size range 201-225 forward primer 148- > 169: AACTTCTGATTCGTCCAGAAGC Tm = 59.9 reverse primer 354->334: TGATCTTGTTTCTGGGTTTGG Tm = 60.0 PCR product length: 207, GC = 42% 34493 Product size range 151-175 forward primer 216- > 237: ATGGATACTGGATCGATGTTCC Tm = 60.0 reverse primer 378->359: TGCACAAAGAAACAAGCGAC Tm = 60.0 PCR product length: 163, GC = 44% 34356 Product size range 351-400 forward primer 61->82: ACCATCGTTTCTCTAATCTCCG Tm = 59.6 reverse primer 418- > 397: CAGGAGCACAACATATAGCTGC Tm = 59.9 PCR product length: 358, GC = 49% 34006 Product size range 326-350 forward primer 90->110: TTTATTCTGCCGACAACAACC Tm = 60.0 reverse primer 433->414: TCCGAAGAGGAACACTTTGC Tm = 60.4 PCR product length: 344, GC =50% 34397 Product size range 301-325 forward primer 43->61: CCAGCGAGTCTTTACGCAG Tm = 59.7 reverse primer 361- > 344: AGCCTTGTCCCTCGCTTC Tm = 60.5 PCR product length: 319, GC =50%