{plate 17 forward} {1-97840} {Product size range 251-275...} {forward primer 93->110} ACCGGAAAGGCCGTTAAG {Tm = 60.1} {PCR product length: 254, GC = 61%} {2-97841} {Product size range 201-225...} {forward primer 56->75 } GCCTCAACAATGGCTCTCTC {Tm = 60.0} {PCR product length: 214, GC = 58%} {3-97843} {Product size range 151-200...} {forward primer 134->153} TGGTCCTCTTCCTGAGCAGT {Tm = 60.0} {PCR product length: 163, GC = 44%} {4-97844} {Product size range 201-225...} {forward primer 153->172} AATGGCGACTGGAGTATTGC {Tm = 60.1} {PCR product length: 225, GC = 47%} {5-97847} {Product size range 226-250...} {forward primer 105->125} AACAAAGGTACTGTGGATGCG {Tm = 60.0} {PCR product length: 229, GC = 46%} {6-97852} {Product size range 151-200...} {forward primer 58->77 } TTCGTCTCCCATATTCTCCG {Tm = 60.0} {PCR product length: 196, GC = 43%} {7-97855} {Product size range 100-125...} {forward primer 109->126} AAGGAACCGGACCAGGAG {Tm = 60.0} {PCR product length: 102, GC = 48%} {8-97859} {Product size range 201-225...} {forward primer 70->91 } GATAATGACCTCAACCAATCCG {Tm = 60.6} {PCR product length: 209, GC = 48%} {9-97860} {Product size range 201-225...} {forward primer 125->144} ACACCACGACAAGACAGCAG {Tm = 59.9} {PCR product length: 214, GC = 53%} {10-97864} {Product size range 251-275...} {forward primer 79->99 } GTTCTAGCCTTCACGATGTGC {Tm = 59.9} {PCR product length: 251, GC = 47%} {11-97867} {Product size range 226-250...} {forward primer 85->102} CCAGCCCAAGGTCACATC {Tm = 60.0} {PCR product length: 236, GC = 53%} {12-97868} {Product size range 251-275...} {forward primer 121->140} TTGCGATCGTTGAAGATCTG {Tm = 59.9} {PCR product length: 265, GC = 44%} {13-97870} {Product size range 201-225...} {forward primer 79->96 } ACTGGAGCTGTTGTCCGC {Tm = 60.0} {PCR product length: 216, GC = 48%} {14-97872} {Product size range 251-275...} {forward primer 63->83 } CTGCTCGTCGCACTAGTTACA {Tm = 59.3} {PCR product length: 263, GC = 46%} {15-97873} {Product size range 301-325...} {forward primer 177->196} GGTATTGGAGCTGGCATTGT {Tm = 60.0} {PCR product length: 305, GC = 44%} {16-97874} {Product size range 151-200...} {forward primer 70->89 } CTGACAAGACCCAGTGCGTA {Tm = 59.9} {PCR product length: 157, GC = 54%} {17-97876} {Product size range 251-275...} {forward primer 69->88 } GAACACGGCATTGATCACAC {Tm = 60.0} {PCR product length: 263, GC = 48%} {18-97877} {Product size range 100-125...} {forward primer 161->182} AGAACTTGACCACACATTGACG {Tm = 60.1} {PCR product length: 106, GC = 43%} {19-97878} {Product size range 276-300...} {forward primer 92->111} TCAGCAAGGAGCTTAGCCTC {Tm = 59.9} {PCR product length: 277, GC = 48%} {20-97881} {Product size range 251-275...} {forward primer 53->70 } AATCTGGCTGGTTCGCTG {Tm = 59.9} {PCR product length: 252, GC = 45%} {21-97886} {Product size range 251-275...} {forward primer 96->113} GGGACGATGGATCGACAC {Tm = 59.8} {PCR product length: 261, GC = 47%} {22-97887} {Product size range 326-350...} {forward primer 52->69 } GGCAGACTCGACGAGCAT {Tm = 60.1} {PCR product length: 327, GC = 52%} {23-97889} {Product size range 126-150...} {forward primer 131->149} ACGATGAGTACAGGCGCAG {Tm = 60.0} {PCR product length: 128, GC = 52%} {24-97890} {Product size range 100-125...} {forward primer 90->110} TACACGAAACCATTCACCACA {Tm = 59.9} {PCR product length: 125, GC = 52%} {25-97891} {Product size range 326-350...} {forward primer 64->82 } AACAGCAGCGGTTGTTGAG {Tm = 60.0} {PCR product length: 342, GC = 47%} {26-97892} {Product size range 126-150...} {forward primer 170->190} AGCAACGGAGGAAGAGTTAGC {Tm = 60.0} {PCR product length: 139, GC = 47%} {27-97897} {Product size range 301-325...} {forward primer 109->126} ACCGCTGGAACAGAGCAC {Tm = 60.0} {PCR product length: 302, GC = 51%} {28-97898} {Product size range 100-125...} {forward primer 252->271} CCCGGCTTCATACATTGAGT {Tm = 60.0} {PCR product length: 113, GC = 48%} {29-97899} {Product size range 100-125...} {forward primer 207->226} GAGGAGGCTGCTAAGGACCT {Tm = 60.0} {PCR product length: 114, GC = 42%} {30-97900} {Product size range 201-225...} {forward primer 138->157} AGCTCGTCGCTGTTAACGAT {Tm = 60.0} {PCR product length: 204, GC = 47%} {31-97904} {Product size range 251-275...} {forward primer 100->117} TAGCCGGTTTAGTTGGCG {Tm = 59.8} {PCR product length: 253, GC = 50%} {32-97907} {Product size range 276-300...} {forward primer 140->160} GAGCATCATCAAGATGAAGGC {Tm = 59.8} {PCR product length: 284, GC = 46%} {33-97909} {Product size range 201-225...} {forward primer 81->100} TCTCCAATTGCAATGCAGAG {Tm = 59.9} {PCR product length: 218, GC = 46%} {34-97911} {Product size range 100-125...} {forward primer 222->242} ATGATCGTGGAACAGAAGGTG {Tm = 60.0} {PCR product length: 120, GC = 42%} {35-97912} {Product size range 201-225...} {forward primer 118->139} CGCTACAACCTCTGTACTCACG {Tm = 60.0} {PCR product length: 201, GC = 50%} {36-97916} {Product size range 226-250...} {forward primer 64->83 } TGCATCACCGAAGCTTACAG {Tm = 60.0} {PCR product length: 243, GC = 48%} {37-97918} {Product size range 226-250...} {forward primer 87->106} GTCGTCTCAGAAGTAGCCGC {Tm = 60.2} {PCR product length: 230, GC = 50%} {38-97919} {Product size range 201-225...} {forward primer 118->139} CGCTACAACCTCTGTACTCACG {Tm = 60.0} {PCR product length: 201, GC = 50%} {39-97921} {Product size range 251-275...} {forward primer 58->77 } CACGTAGATCGAGTGCCTTG {Tm = 59.5} {PCR product length: 256, GC = 46%} {40-97922} {Product size range 126-150...} {forward primer 193->212} CAAGGATCCTCAGGCTTCTG {Tm = 59.9} {PCR product length: 127, GC = 44%} {41-97923} {Product size range 151-200...} {forward primer 92->113} GAATCGAACAAACCTGTTAGGC {Tm = 60.0} {PCR product length: 180, GC = 43%} {42-97926} {Product size range 326-350...} {forward primer 79->97 } CTTTCGAGGTTGAGGAGGC {Tm = 59.9} {PCR product length: 331, GC = 51%} {43-97927} {Product size range 201-225...} {forward primer 70->90 } GAGTTCTCATCGCTCTTCACG {Tm = 60.1} {PCR product length: 223, GC = 42%} {44-97928} {Product size range 126-150...} {forward primer 221->240} TCAGCGAGTCTTGCTAACGA {Tm = 59.9} {PCR product length: 150, GC = 49%} {45-97930} {Product size range 151-200...} {forward primer 227->245} GGTCCAGCTCTCGGTCTTC {Tm = 59.9} {PCR product length: 165, GC = 48%} {46-97931} {Product size range 201-225...} {forward primer 128->146} AACTCCTCCGATCTCACCG {Tm = 60.2} {PCR product length: 208, GC = 39%} {47-97935} {Product size range 226-250...} {forward primer 172->191} TCTCTGTGGGTAGTGGAGGC {Tm = 60.3} {PCR product length: 236, GC = 42%} {48-97937} {Product size range 100-125...} {forward primer 228->246} AACGGGTCAGGATCAGGAG {Tm = 60.1} {PCR product length: 124, GC = 52%} {49-97938} {Product size range 226-250...} {forward primer 154->171} TCTCTTCCTTGATCGGCG {Tm = 60.0} {PCR product length: 238, GC = 49%} {50-97939} {Product size range 201-225...} {forward primer 103->122} GTGCATTTCGATCCACATTG {Tm = 59.9} {PCR product length: 217, GC = 49%} {51-97943} {Product size range 226-250...} {forward primer 138->155} GGAACCGTTGATTCGCAC {Tm = 60.1} {PCR product length: 247, GC = 57%} {52-97944} {Product size range 126-150...} {forward primer 93->112} CTCAGTGGGCTTGGTTCTTC {Tm = 59.8} {PCR product length: 136, GC = 57%} {53-97946} {Product size range 276-300...} {forward primer 52->73 } TAGGGTTAAGACCACAGAGGTG {Tm = 58.3} {PCR product length: 299, GC = 41%} {54-97948} {Product size range 276-300...} {forward primer 55->76 } CTAGGATTCGAAGAGTTCGAGC {Tm = 59.6} {PCR product length: 290, GC = 43%} {55-97953} {Product size range 251-275...} {forward primer 57->76 } AGAACCATCCGTCGTCAATC {Tm = 59.9} {PCR product length: 269, GC = 43%} {56-97954} {Product size range 301-325...} {forward primer 51->70 } GACTCTGCCACGTTCTCCTC {Tm = 60.0} {PCR product length: 324, GC = 53%} {57-97958} {Product size range 151-200...} {forward primer 74->93 } CGGAAACCTAGGATTCGTCA {Tm = 60.1} {PCR product length: 152, GC = 46%} {58-97960} {Product size range 251-275...} {forward primer 74->95 } TCCTTAAATCGCTTCTCTCTCG {Tm = 60.1} {PCR product length: 252, GC = 46%} {59-97963} {Product size range 226-250...} {forward primer 66->86 } GACGTTTGGGCTATTCCTAGC {Tm = 60.1} {PCR product length: 235, GC = 51%} {60-97964} {Product size range 276-300...} {forward primer 77->96 } AAGCCTCATGCTGAAGGAGA {Tm = 60.1} {PCR product length: 282, GC = 42%} {61-97966} {Product size range 276-300...} {forward primer 74->94 } GAAACACAACAACCATCATCG {Tm = 58.9} {PCR product length: 279, GC = 48%} {62-97969} {Product size range 226-250...} {forward primer 109->129} CGTCAAGAAACCTTGAAGACG {Tm = 59.9} {PCR product length: 232, GC = 48%} {63-97972} {Product size range 201-225...} {forward primer 125->144} TCGAGTTCCTTCAAGAGCGT {Tm = 60.1} {PCR product length: 215, GC = 48%} {64-97975} {Product size range 276-300...} {forward primer 56->75 } AAGTCCACCAAGTCGAATGC {Tm = 60.1} {PCR product length: 300, GC = 43%} {65-97979} {Product size range 226-250...} {forward primer 68->87 } AACTGTGGATGTGGCTCCTC {Tm = 60.1} {PCR product length: 247, GC = 41%} {66-97980} {Product size range 100-125...} {forward primer 142->162} TGATGCAGCTGTATGTTCAGC {Tm = 60.0} {PCR product length: 112, GC = 41%} {67-97982} {Product size range 226-250...} {forward primer 160->178} CGTCCTCTAGGCTTCTCCG {Tm = 60.1} {PCR product length: 232, GC = 50%} {68-97987} {Product size range 276-300...} {forward primer 69->87 } GCAGAGCAGGGAGAGGATC {Tm = 60.0} {PCR product length: 291, GC = 46%} {69-97989} {Product size range 276-300...} {forward primer 75->93 } TTCAGATGCTCACCGGAAG {Tm = 59.9} {PCR product length: 281, GC = 49%} {70-97990} {Product size range 276-300...} {forward primer 91->110} AGGAAGAGGAAGAGGTTCGC {Tm = 60.0} {PCR product length: 277, GC = 36%} {71-97991} {Product size range 226-250...} {forward primer 112->130} GGACAGCCAGTTCTTGCAG {Tm = 59.6} {PCR product length: 242, GC = 43%} {72-97994} {Product size range 326-350...} {forward primer 111->131} AATCTCCAATCAAGCGACTCA {Tm = 59.8} {PCR product length: 348, GC = 47%} {73-97995} {Product size range 151-200...} {forward primer 121->138} TTTCTTCCGCAGTGGCTC {Tm = 60.1} {PCR product length: 171, GC = 57%} {74-97996} {Product size range 151-200...} {forward primer 226->245} GTTGGAGTTCTCAAGCCGAG {Tm = 60.0} {PCR product length: 154, GC = 49%} {75-97998} {Product size range 151-200...} {forward primer 161->181} AAAGAACGAGTTCTTCGAGGC {Tm = 60.0} {PCR product length: 169, GC = 46%} {76-97999} {Product size range 126-150...} {forward primer 200->217} CGTGTCTCACTGCGGTTG {Tm = 60.0} {PCR product length: 146, GC = 52%} {77-98002} {Product size range 226-250...} {forward primer 153->172} CATGGCTTTCAACAAGCTCA {Tm = 60.0} {PCR product length: 249, GC = 53%} {78-98003} {Product size range 151-200...} {forward primer 112->131} GTTCTTCTCTCCGTCAACGC {Tm = 60.0} {PCR product length: 196, GC = 55%} {79-98004} {Product size range 301-325...} {forward primer 99->118} TTTACGGCTCTATGGTTCCG {Tm = 60.1} {PCR product length: 321, GC = 46%} {80-98006} {Product size range 126-150...} {forward primer 220->239} CCCTCTCACAGTTCCTCCTG {Tm = 59.8} {PCR product length: 133, GC = 57%} {81-98007} {Product size range 126-150...} {forward primer 215->234} CACCAGGTATGTGGATGCTG {Tm = 60.0} {PCR product length: 142, GC = 48%} {82-98011} {Product size range 100-125...} {forward primer 122->141} GCTGTTCTTGCACAAGGTGA {Tm = 60.0} {PCR product length: 115, GC = 45%} {83-98015} {Product size range 100-125...} {forward primer 136->153} ACCAGTTGCGTCTGCCTC {Tm = 60.0} {PCR product length: 106, GC = 49%} {84-98017} {Product size range 226-250...} {forward primer 62->80 } CTCCGCAATGTCATTGGTC {Tm = 60.1} {PCR product length: 232, GC = 51%} {85-98019} {Product size range 201-225...} {forward primer 126->145} AATTCCATGTCATCGGTGGT {Tm = 60.1} {PCR product length: 203, GC = 48%} {86-98023} {Product size range 251-275...} {forward primer 72->91 } TAGCTTTCGCCGAAGATGAT {Tm = 59.9} {PCR product length: 252, GC = 46%} {87-98024} {Product size range 151-200...} {forward primer 109->127} TGGTTGCGGCTACTACTGC {Tm = 60.0} {PCR product length: 171, GC = 45%} {88-98026} {Product size range 151-200...} {forward primer 123->140} CCATTGAGGCTGGTGGTC {Tm = 60.0} {PCR product length: 193, GC = 49%} {89-98029} {Product size range 226-250...} {forward primer 50->69 } TCTGTCTGACTTCAATGGCG {Tm = 60.0} {PCR product length: 226, GC = 44%} {90-98031} {Product size range 126-150...} {forward primer 169->187} ACGGTTATCTCAGACCGGC {Tm = 60.1} {PCR product length: 128, GC = 52%} {91-98032} {Product size range 301-325...} {forward primer 159->178} CATCGATCCGAGTAGGACGT {Tm = 60.1} {PCR product length: 320, GC = 44%} {92-98033} {Product size range 100-125...} {forward primer 60->80 } AAGAAGAAGAAGTTCTCGGCG {Tm = 60.1} {PCR product length: 105, GC = 53%} {93-98034} {Product size range 251-275...} {forward primer 86->105} ACTGGTTTGAAGTCATCCGC {Tm = 60.1} {PCR product length: 269, GC = 49%} {94-98036} {Product size range 201-225...} {forward primer 138->157} GTGGTGACAGTGGTTCGATG {Tm = 60.0} {PCR product length: 221, GC = 44%} {95-98037} {Product size range 276-300...} {forward primer 71->89 } TTTGCCACTTACGAGGCTG {Tm = 60.0} {PCR product length: 298, GC = 49%} {96-98042} {Product size range 276-300...} {forward primer 75->94 } TGGCAGTCTTCCGAAGTACA {Tm = 59.4} {PCR product length: 300, GC = 50%} {plate 17 reverse} {1-97840} {Product size range 251-275...} {reverse primer 346->327} CTAGCTCACGGTTCCTAGCG {Tm = 60.2} {PCR product length: 254, GC = 61%} {2-97841} {Product size range 201-225...} {reverse primer 269->249} ACTCTCCGGTAAGGTAGCTCG {Tm = 59.9} {PCR product length: 214, GC = 58%} {3-97843} {Product size range 151-200...} {reverse primer 296->277} CCGGAAACAAACTCTAGCCA {Tm = 60.2} {PCR product length: 163, GC = 44%} {4-97844} {Product size range 201-225...} {reverse primer 377->358} CCTGCCTTGGCACTAAGAAG {Tm = 60.0} {PCR product length: 225, GC = 47%} {5-97847} {Product size range 226-250...} {reverse primer 333->316} GGTCTCCGCCCTCTTAGC {Tm = 59.9} {PCR product length: 229, GC = 46%} {6-97852} {Product size range 151-200...} {reverse primer 253->234} CATTCACATCCCATTCACCA {Tm = 60.2} {PCR product length: 196, GC = 43%} {7-97855} {Product size range 100-125...} {reverse primer 210->191} TGTCAACAGGAACACGTGGT {Tm = 60.0} {PCR product length: 102, GC = 48%} {8-97859} {Product size range 201-225...} {reverse primer 278->257} CACGATCAAACAATTGAAGAGC {Tm = 59.8} {PCR product length: 209, GC = 48%} {9-97860} {Product size range 201-225...} {reverse primer 338->321} CACCGGATGCTCCGTTAC {Tm = 60.1} {PCR product length: 214, GC = 53%} {10-97864} {Product size range 251-275...} {reverse primer 329->311} CTCTGGTTTCTCTGGCGGT {Tm = 60.4} {PCR product length: 251, GC = 47%} {11-97867} {Product size range 226-250...} {reverse primer 320->301} ATGAAGTTTCCAGTGCCGTC {Tm = 60.1} {PCR product length: 236, GC = 53%} {12-97868} {Product size range 251-275...} {reverse primer 385->366} ATTCACCCAAAGTCAGCCAC {Tm = 60.0} {PCR product length: 265, GC = 44%} {13-97870} {Product size range 201-225...} {reverse primer 294->275} TCACAGGGTTGCTGATCAAG {Tm = 59.8} {PCR product length: 216, GC = 48%} {14-97872} {Product size range 251-275...} {reverse primer 325->308} ACTTGCGGTACTGTCCGC {Tm = 59.9} {PCR product length: 263, GC = 46%} {15-97873} {Product size range 301-325...} {reverse primer 481->464} CCCAAGGTGGCAATTACG {Tm = 59.9} {PCR product length: 305, GC = 44%} {16-97874} {Product size range 151-200...} {reverse primer 226->209} TTGACGCAGCTGCAAGAG {Tm = 60.0} {PCR product length: 157, GC = 54%} {17-97876} {Product size range 251-275...} {reverse primer 331->311} CAATTCAACTTCAAGGCTTCG {Tm = 59.9} {PCR product length: 263, GC = 48%} {18-97877} {Product size range 100-125...} {reverse primer 266->247} TCAGCTGATCGTTCTTGTGC {Tm = 60.1} {PCR product length: 106, GC = 43%} {19-97878} {Product size range 276-300...} {reverse primer 368->351} CCCTTCACCCACAGCAAC {Tm = 60.1} {PCR product length: 277, GC = 48%} {20-97881} {Product size range 251-275...} {reverse primer 304->285} AAAGGCAGAAGTCAAGGCAG {Tm = 59.6} {PCR product length: 252, GC = 45%} {21-97886} {Product size range 251-275...} {reverse primer 356->337} GCCTTCCTTGTTGGAAGATG {Tm = 59.7} {PCR product length: 261, GC = 47%} {22-97887} {Product size range 326-350...} {reverse primer 378->360} TTTCGAGAGCTCCGTGATG {Tm = 60.1} {PCR product length: 327, GC = 52%} {23-97889} {Product size range 126-150...} {reverse primer 258->238} AGGAATCTAGCGATTCTTCCG {Tm = 59.8} {PCR product length: 128, GC = 52%} {24-97890} {Product size range 100-125...} {reverse primer 214->196} TTTGCGCTGTTGTAGGCTC {Tm = 60.1} {PCR product length: 125, GC = 52%} {25-97891} {Product size range 326-350...} {reverse primer 405->386} GGAAGGAAGTCCGTCATTGA {Tm = 60.0} {PCR product length: 342, GC = 47%} {26-97892} {Product size range 126-150...} {reverse primer 308->290} TCCACTTGTTGCGGAGAAG {Tm = 60.0} {PCR product length: 139, GC = 47%} {27-97897} {Product size range 301-325...} {reverse primer 410->390} TTTATTAACGGGACAACCACG {Tm = 59.7} {PCR product length: 302, GC = 51%} {28-97898} {Product size range 100-125...} {reverse primer 364->347} CGCTAGCTTGCTCCAAGC {Tm = 60.0} {PCR product length: 113, GC = 48%} {29-97899} {Product size range 100-125...} {reverse primer 320->300} AGCCCTTTCAAGTTCTCTTGC {Tm = 60.0} {PCR product length: 114, GC = 42%} {30-97900} {Product size range 201-225...} {reverse primer 341->322} AAAGTCAGCTCCAAGCCTCA {Tm = 60.1} {PCR product length: 204, GC = 47%} {31-97904} {Product size range 251-275...} {reverse primer 352->333} CACGAGAAAGTTAGCCGGAG {Tm = 60.0} {PCR product length: 253, GC = 50%} {32-97907} {Product size range 276-300...} {reverse primer 423->405} AACCAAGGACCAGCGGTAC {Tm = 60.0} {PCR product length: 284, GC = 46%} {33-97909} {Product size range 201-225...} {reverse primer 298->278} GAAGCTCCTCTCCTAAGCTGC {Tm = 59.9} {PCR product length: 218, GC = 46%} {34-97911} {Product size range 100-125...} {reverse primer 341->320} TTGATGAAGATTCTGGTTGACG {Tm = 60.1} {PCR product length: 120, GC = 42%} {35-97912} {Product size range 201-225...} {reverse primer 318->298} ATATCCACAGCAACCTGATCG {Tm = 60.0} {PCR product length: 201, GC = 50%} {36-97916} {Product size range 226-250...} {reverse primer 306->287} TTGGGATCTCCGTCTTCATC {Tm = 60.0} {PCR product length: 243, GC = 48%} {37-97918} {Product size range 226-250...} {reverse primer 316->298} TGGTACCGTCCTCCTCCTC {Tm = 60.1} {PCR product length: 230, GC = 50%} {38-97919} {Product size range 201-225...} {reverse primer 318->298} ATATCCACAGCAACCTGATCG {Tm = 60.0} {PCR product length: 201, GC = 50%} {39-97921} {Product size range 251-275...} {reverse primer 313->294} ATGGTGAAAGGAACGAGTCG {Tm = 60.1} {PCR product length: 256, GC = 46%} {40-97922} {Product size range 126-150...} {reverse primer 319->300} AAGCTCCTGGTGCACTTCAT {Tm = 59.9} {PCR product length: 127, GC = 44%} {41-97923} {Product size range 151-200...} {reverse primer 271->251} TCTACTGGAAGAAGCTGCTGC {Tm = 59.9} {PCR product length: 180, GC = 43%} {42-97926} {Product size range 326-350...} {reverse primer 409->390} TACCCGCAACTGTCTGACAT {Tm = 59.2} {PCR product length: 331, GC = 51%} {43-97927} {Product size range 201-225...} {reverse primer 292->273} GAGCCAAGGGAGACAAGTTG {Tm = 59.8} {PCR product length: 223, GC = 42%} {44-97928} {Product size range 126-150...} {reverse primer 370->350} ATGATGAAACACGTGTCCACA {Tm = 59.9} {PCR product length: 150, GC = 49%} {45-97930} {Product size range 151-200...} {reverse primer 391->371} GCATACAAGGCTGAGATCAGC {Tm = 60.0} {PCR product length: 165, GC = 48%} {46-97931} {Product size range 201-225...} {reverse primer 335->315} TGTCAAGCATCATGTTAAGCG {Tm = 59.9} {PCR product length: 208, GC = 39%} {47-97935} {Product size range 226-250...} {reverse primer 407->389} AATTAAGACCCGCCTGGTG {Tm = 59.9} {PCR product length: 236, GC = 42%} {48-97937} {Product size range 100-125...} {reverse primer 351->332} AGCCAACACCCTTGAATGAC {Tm = 60.0} {PCR product length: 124, GC = 52%} {49-97938} {Product size range 226-250...} {reverse primer 391->372} AGCACTCGGTTTACCAGGTG {Tm = 60.2} {PCR product length: 238, GC = 49%} {50-97939} {Product size range 201-225...} {reverse primer 319->300} CGATCACAGTTGGCTCAAGA {Tm = 60.0} {PCR product length: 217, GC = 49%} {51-97943} {Product size range 226-250...} {reverse primer 384->365} GCCGCTCGGTGGTAATTATA {Tm = 59.9} {PCR product length: 247, GC = 57%} {52-97944} {Product size range 126-150...} {reverse primer 228->211} TCTTAGGCTGAGCAGCCG {Tm = 60.4} {PCR product length: 136, GC = 57%} {53-97946} {Product size range 276-300...} {reverse primer 350->331} CCTTTAAGGCCGCTCTACCT {Tm = 59.9} {PCR product length: 299, GC = 41%} {54-97948} {Product size range 276-300...} {reverse primer 344->325} GGGATTGTGTTGCAAGGAGT {Tm = 60.0} {PCR product length: 290, GC = 43%} {55-97953} {Product size range 251-275...} {reverse primer 325->304} CATAGGTCTCAACTTGCTTCCT {Tm = 57.6} {PCR product length: 269, GC = 43%} {56-97954} {Product size range 301-325...} {reverse primer 374->355} CCATCCGGTAAGATCCAAGA {Tm = 59.9} {PCR product length: 324, GC = 53%} {57-97958} {Product size range 151-200...} {reverse primer 225->207} TCGGAGCATCAATCTCGAG {Tm = 60.0} {PCR product length: 152, GC = 46%} {58-97960} {Product size range 251-275...} {reverse primer 325->305} CACAGCAGACGTACCTTGACA {Tm = 60.0} {PCR product length: 252, GC = 46%} {59-97963} {Product size range 226-250...} {reverse primer 300->281} AGCGGAGAAGACGGTGTAGA {Tm = 60.0} {PCR product length: 235, GC = 51%} {60-97964} {Product size range 276-300...} {reverse primer 358->337} GAAATGTTTAAAGGCAATCCAG {Tm = 57.8} {PCR product length: 282, GC = 42%} {61-97966} {Product size range 276-300...} {reverse primer 352->333} AGAGGGTAGCCACCGAGAGT {Tm = 60.3} {PCR product length: 279, GC = 48%} {62-97969} {Product size range 226-250...} {reverse primer 340->320} TCTATAAACCCGTCTCCGTCA {Tm = 59.6} {PCR product length: 232, GC = 48%} {63-97972} {Product size range 201-225...} {reverse primer 339->321} AGACACGAGTGGTGCAAGC {Tm = 60.0} {PCR product length: 215, GC = 48%} {64-97975} {Product size range 276-300...} {reverse primer 355->334} AAGGCCAGTACAAGTAAGCTGC {Tm = 60.0} {PCR product length: 300, GC = 43%} {65-97979} {Product size range 226-250...} {reverse primer 314->295} TTGATACGCAACACAACGGT {Tm = 60.0} {PCR product length: 247, GC = 41%} {66-97980} {Product size range 100-125...} {reverse primer 253->232} ACGATCATCTTGACCAACTTCA {Tm = 59.6} {PCR product length: 112, GC = 41%} {67-97982} {Product size range 226-250...} {reverse primer 391->372} CGGATTCTGGTACCGTCAGT {Tm = 60.0} {PCR product length: 232, GC = 50%} {68-97987} {Product size range 276-300...} {reverse primer 359->340} TTAAAGCTCTAGGTCCGGCA {Tm = 60.0} {PCR product length: 291, GC = 46%} {69-97989} {Product size range 276-300...} {reverse primer 355->336} TCAAAGACAGCTAGCCGACA {Tm = 59.7} {PCR product length: 281, GC = 49%} {70-97990} {Product size range 276-300...} {reverse primer 367->348} AAGCCAACCCAACATCACTC {Tm = 60.0} {PCR product length: 277, GC = 36%} {71-97991} {Product size range 226-250...} {reverse primer 353->335} AAAGCTGCGGGAAGACAAC {Tm = 60.4} {PCR product length: 242, GC = 43%} {72-97994} {Product size range 326-350...} {reverse primer 458->440} TTTAACCTCAGGAAGCCGC {Tm = 60.3} {PCR product length: 348, GC = 47%} {73-97995} {Product size range 151-200...} {reverse primer 291->271} GTGGTGAAGCAGAGGATTCTG {Tm = 59.9} {PCR product length: 171, GC = 57%} {74-97996} {Product size range 151-200...} {reverse primer 379->360} GTGTTGCCAATGGAGGAACT {Tm = 60.0} {PCR product length: 154, GC = 49%} {75-97998} {Product size range 151-200...} {reverse primer 329->308} GAGAAGATCAATGACACGCTTG {Tm = 59.9} {PCR product length: 169, GC = 46%} {76-97999} {Product size range 126-150...} {reverse primer 345->326} GTCTAAACCGAAGCTCCACG {Tm = 59.9} {PCR product length: 146, GC = 52%} {77-98002} {Product size range 226-250...} {reverse primer 401->382} CCGAATTTAAGGATGAGGCA {Tm = 60.0} {PCR product length: 249, GC = 53%} {78-98003} {Product size range 151-200...} {reverse primer 307->288} AAGAACCCTAATCCGCCACT {Tm = 60.0} {PCR product length: 196, GC = 55%} {79-98004} {Product size range 301-325...} {reverse primer 419->400} TGCCTTCAGTGACATGCTTC {Tm = 60.0} {PCR product length: 321, GC = 46%} {80-98006} {Product size range 126-150...} {reverse primer 352->335} ACGAACCGCAGCTTCAAC {Tm = 60.0} {PCR product length: 133, GC = 57%} {81-98007} {Product size range 126-150...} {reverse primer 356->337} CGACCAAATAGGCTGACCAT {Tm = 60.0} {PCR product length: 142, GC = 48%} {82-98011} {Product size range 100-125...} {reverse primer 236->216} GCTTGTCAAACTCCATGAAGC {Tm = 59.9} {PCR product length: 115, GC = 45%} {83-98015} {Product size range 100-125...} {reverse primer 241->222} CGATGAAGCAAACCACAATG {Tm = 60.1} {PCR product length: 106, GC = 49%} {84-98017} {Product size range 226-250...} {reverse primer 293->273} AGGAAAGATTCCAGAACCAGC {Tm = 59.7} {PCR product length: 232, GC = 51%} {85-98019} {Product size range 201-225...} {reverse primer 328->309} CATCATCATCAGATCACGGC {Tm = 60.0} {PCR product length: 203, GC = 48%} {86-98023} {Product size range 251-275...} {reverse primer 323->304} GGTTTAAGGCACAGATCGGA {Tm = 60.1} {PCR product length: 252, GC = 46%} {87-98024} {Product size range 151-200...} {reverse primer 279->260} CCCATTTAGCAACAGGACGT {Tm = 60.0} {PCR product length: 171, GC = 45%} {88-98026} {Product size range 151-200...} {reverse primer 315->296} ATGTTACGCGCCATCCTTAG {Tm = 60.1} {PCR product length: 193, GC = 49%} {89-98029} {Product size range 226-250...} {reverse primer 275->256} GGGCAATTGTTTGGCAATAG {Tm = 60.3} {PCR product length: 226, GC = 44%} {90-98031} {Product size range 126-150...} {reverse primer 296->278} AATTGGAGATACTCCGCCG {Tm = 60.0} {PCR product length: 128, GC = 52%} {91-98032} {Product size range 301-325...} {reverse primer 478->459} GAATTTGCCCTACCACAGGA {Tm = 59.9} {PCR product length: 320, GC = 44%} {92-98033} {Product size range 100-125...} {reverse primer 164->147} GAGCAACGGGTGGATACG {Tm = 60.1} {PCR product length: 105, GC = 53%} {93-98034} {Product size range 251-275...} {reverse primer 354->336} AGTTGTTTCCGTGCTCACG {Tm = 59.9} {PCR product length: 269, GC = 49%} {94-98036} {Product size range 201-225...} {reverse primer 358->339} CCCGATAAGCAGATTCAGGA {Tm = 60.2} {PCR product length: 221, GC = 44%} {95-98037} {Product size range 276-300...} {reverse primer 368->350} CTGCAGGAGCATGGAAGAG {Tm = 59.7} {PCR product length: 298, GC = 49%} {96-98042} {Product size range 276-300...} {reverse primer 374->357} TAAGGCCACTTGAAGGCG {Tm = 59.9} {PCR product length: 300, GC = 50%}