{1-97177} {Product size range 100-150...} {reverse primer 313->293} AGAACAACTTGCACTCCTCCA {Tm = 59.9} {PCR product length: 133, GC = 49%} {2-97178} {Product size range 151-200...} {reverse primer 243->222} ACAAACACAAACGACATGAAGC {Tm = 60.1} {PCR product length: 153, GC = 39%} {3-97188} {Product size range 100-150...} {reverse primer 172->153} TGCGTGATATCCTTCATCGA {Tm = 60.2} {PCR product length: 116, GC = 42%} {4-97193} {Product size range 251-300...} {reverse primer 421->404} CCAGGTTGTAACACCGGC {Tm = 60.0} {PCR product length: 284, GC = 45%} {5-97210} {Product size range 100-150...} {reverse primer 169->150} CCCAGGGCTTCTCAATGATA {Tm = 60.0} {PCR product length: 115, GC = 43%} {6-97212} {Product size range 100-150...} {reverse primer 202->183} AAATCGAGATCGAATCCACG {Tm = 60.0} {PCR product length: 111, GC = 42%} {7-97220} {Product size range 301-350...} {reverse primer 381->360} AAATCCCTTAAGAACCGAGAGA {Tm = 58.4} {PCR product length: 320, GC = 42%} {8-97230} {Product size range 251-300...} {reverse primer 346->327} CGGGATAAAGTTGCACCATC {Tm = 60.3} {PCR product length: 256, GC = 48%} {9-97249} {Product size range 100-150...} {reverse primer 184->166} CGGACGGTGAGTGGTGTAC {Tm = 60.0} {PCR product length: 127, GC = 47%} {10-97260} {Product size range 151-200...} {reverse primer 279->260} CTTCGTCAACCACTTCAGCA {Tm = 60.0} {PCR product length: 187, GC = 45%} {11-97266} {Product size range 301-350...} {reverse primer 385->365} CACTAAGAAAGGTGACGACCG {Tm = 59.8} {PCR product length: 312, GC = 47%} {12-97271} {Product size range 201-250...} {reverse primer 343->323} TGAATTGGTTCATCGTCATCA {Tm = 59.9} {PCR product length: 244, GC = 46%} {13-97276} {Product size range 201-250...} {reverse primer 388->368} ACTAGCCATCTAGGCTCCAGC {Tm = 60.0} {PCR product length: 212, GC = 49%} {14-97277} {Product size range 100-150...} {reverse primer 222->205} GCTTGAACCGCCATGAAC {Tm = 60.2} {PCR product length: 137, GC = 34%} {15-97278} {Product size range 100-150...} {reverse primer 168->149} CAGCTGCCACCACATTAATG {Tm = 60.1} {PCR product length: 105, GC = 48%} {16-97287} {Product size range 251-300...} {reverse primer 332->313} TGACAAATATCGTCTTGCGC {Tm = 59.8} {PCR product length: 269, GC = 43%} {17-97304} {Product size range 251-300...} {reverse primer 329->310} TGCCTCTTTAGCTCGGAGAC {Tm = 59.7} {PCR product length: 266, GC = 43%} {18-97312} {Product size range 100-150...} {reverse primer 169->150} CGGAGAGAGTGGAGACCAAG {Tm = 60.0} {PCR product length: 118, GC = 48%} {19-97316} {Product size range 201-250...} {reverse primer 321->303} AGCACCGAATGTGTTCTCG {Tm = 59.8} {PCR product length: 202, GC = 43%} {20-97318} {Product size range 100-150...} {reverse primer 248->229} TAGCTTGCTCAGCGATCTCA {Tm = 60.0} {PCR product length: 101, GC = 44%} {21-97325} {Product size range 201-250...} {reverse primer 348->329} CTGGACTCTCACCGCTAAGC {Tm = 60.2} {PCR product length: 215, GC = 49%} {22-97326} {Product size range 251-300...} {reverse primer 331->312} ATTCTGGCAAGCTCTCAGGA {Tm = 60.1} {PCR product length: 260, GC = 43%} {23-97330} {Product size range 100-150...} {reverse primer 225->206} GTTAATTGAGCGTGGACGGT {Tm = 60.0} {PCR product length: 139, GC = 45%} {24-97341} {Product size range 100-150...} {reverse primer 191->172} CATCTGGATTTGACGTGGTG {Tm = 60.0} {PCR product length: 113, GC = 48%} {25-97343} {Product size range 201-250...} {reverse primer 259->240} GTTGGTGCCCTTGATGGTAT {Tm = 59.7} {PCR product length: 202, GC = 45%} {26-97352} {Product size range 351-400...} {reverse primer 438->419} GGACCACCCAAGTGCCTTAT {Tm = 61.1} {PCR product length: 351, GC = 45%} {27-97356} {Product size range 151-200...} {reverse primer 266->247} CCATACTCGCCAGGAATGAT {Tm = 59.9} {PCR product length: 153, GC = 43%} {28-97357} {Product size range 401-450...} {reverse primer 485->466} GCAATTGAACCTAGGCAGGA {Tm = 60.2} {PCR product length: 412, GC = 40%} {29-97361} {Product size range 100-150...} {reverse primer 234->215} AGACTCGAGCGTCGTTGTCT {Tm = 60.2} {PCR product length: 100, GC = 49%} {30-97367} {Product size range 100-150...} {reverse primer 239->218} CTTTGGGAGAGTGTAACCTTCG {Tm = 60.2} {PCR product length: 145, GC = 44%} {31-97370} {Product size range 151-200...} {reverse primer 268->249} CATACTCCGCCAGGAATGAT {Tm = 59.9} {PCR product length: 155, GC = 43%} {32-97371} {Product size range 201-250...} {reverse primer 326->308} CCTGTGATGACGGTTCCTG {Tm = 60.1} {PCR product length: 201, GC = 45%} {33-97377} {Product size range 251-300...} {reverse primer 372->353} AAGTGGGCTGCCTTCTAGTG {Tm = 59.5} {PCR product length: 252, GC = 47%} {34-97387} {Product size range 151-200...} {reverse primer 253->236} TAAGGTGCCAGCGGAGTC {Tm = 59.9} {PCR product length: 164, GC = 46%} {35-97393} {Product size range 151-200...} {reverse primer 258->237} TGTTTAAATTCGATGATCGACG {Tm = 60.0} {PCR product length: 165, GC = 49%} {36-97418} {Product size range 201-250...} {reverse primer 334->317} CCTTTGCAGGAACAACCG {Tm = 60.2} {PCR product length: 241, GC = 42%} {37-97426} {Product size range 201-250...} {reverse primer 304->285} TCTGCAAACTGCTCTGATGC {Tm = 60.3} {PCR product length: 214, GC = 47%} {38-97431} {Product size range 100-150...} {reverse primer 267->248} AAGACACGGAACCGATGATC {Tm = 59.9} {PCR product length: 102, GC = 46%} {39-97444} {Product size range 100-150...} {reverse primer 228->209} GCACTGCTTCTGATCCATCA {Tm = 59.9} {PCR product length: 137, GC = 49%} {40-97448} {Product size range 151-200...} {reverse primer 216->197} TATACTGAGGCAAGGCGACA {Tm = 59.4} {PCR product length: 156, GC = 36%} {41-97450} {Product size range 100-150...} {reverse primer 209->190} CATGGTACCAGGCTCCAGAT {Tm = 60.0} {PCR product length: 130, GC = 49%} {42-97451} {Product size range 201-250...} {reverse primer 342->325} GCAGGATCCCATGGTCTG {Tm = 60.0} {PCR product length: 222, GC = 48%} {43-97455} {Product size range 151-200...} {reverse primer 215->198} AACGGTGGTCCGTTCTTG {Tm = 59.5} {PCR product length: 153, GC = 49%} {44-97481} {Product size range 100-150...} {reverse primer 261->242} TGCTAATGGGTTGATGAGCA {Tm = 60.2} {PCR product length: 125, GC = 42%} {45-97493} {Product size range 100-150...} {reverse primer 214->195} TTCTCCACCTTCTTCCGAGA {Tm = 59.9} {PCR product length: 135, GC = 47%} {46-97499} {Product size range 201-250...} {reverse primer 271->250} AACGGTTCTTCACCAATGTACA {Tm = 59.4} {PCR product length: 207, GC = 44%} {47-97506} {Product size range 301-350...} {reverse primer 550->533} AACCTGGTAAGGCCGGTC {Tm = 59.9} {PCR product length: 301, GC = 42%} {48-97507} {Product size range 201-250...} {reverse primer 288->271} TACGGTTGATGCCGTCCT {Tm = 60.1} {PCR product length: 206, GC = 48%} {49-97515} {Product size range 151-200...} {reverse primer 250->231} CGTCTTCCAATTGGTTCGAT {Tm = 59.9} {PCR product length: 197, GC = 49%} {50-97522} {Product size range 100-150...} {reverse primer 226->207} GCACTGAGCCTTCCTGAATC {Tm = 60.0} {PCR product length: 128, GC = 48%} {51-97530} {Product size range 151-200...} {reverse primer 309->291} TTGGAGTGCCTTCAACCAG {Tm = 59.8} {PCR product length: 198, GC = 41%} {52-97536} {Product size range 201-250...} {reverse primer 255->236} CCCATCTGTGCCTACCGTAT {Tm = 59.8} {PCR product length: 201, GC = 47%} {53-97547} {Product size range 201-250...} {reverse primer 327->308} AACAGTTAAACCGGTGACCG {Tm = 59.9} {PCR product length: 246, GC = 42%} {54-97553} {Product size range 151-200...} {reverse primer 258->238} ACCACCTTACAGATGCTCTGC {Tm = 59.4} {PCR product length: 184, GC = 41%} {55-97555} {Product size range 201-250...} {reverse primer 315->296} AAGCTGGGAATGATTCGATG {Tm = 60.0} {PCR product length: 250, GC = 46%} {56-97560} {Product size range 100-150...} {reverse primer 305->288} ATGCCCAGCAGTCTGGAG {Tm = 59.9} {PCR product length: 115, GC = 48%} {57-97573} {Product size range 151-200...} {reverse primer 226->208} TCTCGGTCCAATTGAGCTG {Tm = 59.9} {PCR product length: 164, GC = 45%} {58-97574} {Product size range 100-150...} {reverse primer 236->217} AAGCTCCTGCACCGTTCTTA {Tm = 60.0} {PCR product length: 116, GC = 41%} {59-97585} {Product size range 100-150...} {reverse primer 265->246} CCACTGGAGCTTCCTCTGTC {Tm = 60.0} {PCR product length: 106, GC = 47%} {60-97586} {Product size range 100-150...} {reverse primer 265->246} CCACTGGAGCTTCCTCTGTC {Tm = 60.0} {PCR product length: 106, GC = 47%} {61-97602} {Product size range 100-150...} {reverse primer 178->159} ACCACCTTGCCATCTAGCTG {Tm = 60.3} {PCR product length: 100, GC = 44%} {62-97608} {Product size range 151-200...} {reverse primer 227->208} CGACTGTTTCGACAACCATG {Tm = 60.2} {PCR product length: 166, GC = 43%} {63-97611} {Product size range 151-200...} {reverse primer 201->182} TAAACCCTCCATGCCTCATG {Tm = 60.9} {PCR product length: 151, GC = 47%} {64-97623} {Product size range 151-200...} {reverse primer 289->270} TGGTAAATGCCCTCCTTGAG {Tm = 60.1} {PCR product length: 172, GC = 40%} {65-97628} {Product size range 100-150...} {reverse primer 242->223} TTCAACCGGAGCTTCTTCAT {Tm = 59.8} {PCR product length: 144, GC = 44%} {66-97631} {Product size range 201-250...} {reverse primer 300->282} AGCCCAAAGGATCCATCAG {Tm = 60.0} {PCR product length: 216, GC = 48%} {67-97640} {Product size range 151-200...} {reverse primer 289->270} AATCCACGGTACAGACCAGC {Tm = 60.0} {PCR product length: 193, GC = 49%} {68-97655} {Product size range 151-200...} {reverse primer 284->264} CAACCTCTTGATTGCAAGAGC {Tm = 60.0} {PCR product length: 161, GC = 43%} {69-97660} {Product size range 100-150...} {reverse primer 276->258} GGGATCCCACCGACAATAG {Tm = 60.1} {PCR product length: 102, GC = 48%} {70-97663} {Product size range 100-150...} {reverse primer 302->283} TCCAAACTGCGAACACTGAG {Tm = 60.0} {PCR product length: 116, GC = 48%} {71-97673} {Product size range 351-400...} {reverse primer 544->527} CAAATGGGCCCTCAATTG {Tm = 59.9} {PCR product length: 394, GC = 43%} {72-97676} {Product size range 151-200...} {reverse primer 266->245} CAATCGCGCTATAGTAGTGACG {Tm = 60.0} {PCR product length: 181, GC = 45%} {73-97682} {Product size range 100-150...} {reverse primer 449->430} CAGACATTGAAGCCTCACGA {Tm = 60.0} {PCR product length: 149, GC = 48%} {74-97685} {Product size range 100-150...} {reverse primer 195->174} TTTGTCGTATGAAGGAATGTGC {Tm = 60.0} {PCR product length: 101, GC = 42%} {75-97693} {Product size range 100-150...} {reverse primer 224->205} TGTGCTCTTCTTGGCACTTG {Tm = 60.2} {PCR product length: 110, GC = 48%} {76-97709} {Product size range 251-300...} {reverse primer 364->345} GACCAGCCCTCAGTGCATAT {Tm = 60.1} {PCR product length: 287, GC = 45%} {77-97710} {Product size range 100-150...} {reverse primer 211->190} GGTGAAATTAGCAACCTTCTCG {Tm = 60.1} {PCR product length: 119, GC = 47%} {78-97724} {Product size range 100-150...} {reverse primer 353->332} AACTTCACCACTTGACATCACG {Tm = 60.1} {PCR product length: 104, GC = 43%} {79-97732} {Product size range 151-200...} {reverse primer 335->315} TCATCCTCTTCATCCTCCATG {Tm = 60.0} {PCR product length: 193, GC = 44%} {80-97743} {Product size range 301-350...} {reverse primer 375->358} ACCGAGAAAGTGATGCCG {Tm = 59.8} {PCR product length: 301, GC = 43%} {81-97745} {Product size range 151-200...} {reverse primer 293->275} TGATTTCGACTCGCTCCAG {Tm = 60.1} {PCR product length: 160, GC = 46%} {82-97746} {Product size range 151-200...} {reverse primer 321->303} CCACGTTCGGATGATGAAG {Tm = 60.1} {PCR product length: 185, GC = 46%} {83-97757} {Product size range 151-200...} {reverse primer 311->293} TCCACTTGTTGCGGAGAAG {Tm = 60.0} {PCR product length: 162, GC = 48%} {84-97758} {Product size range 100-150...} {reverse primer 202->185} CAACCGCTGCAATCTGTG {Tm = 60.0} {PCR product length: 109, GC = 48%} {85-97760} {Product size range 151-200...} {reverse primer 318->300} TCCACTTGTTGCGGAGAAG {Tm = 60.0} {PCR product length: 162, GC = 48%} {86-97761} {Product size range 251-300...} {reverse primer 343->324} AACCGGAGTTTGAACACCAG {Tm = 60.0} {PCR product length: 277, GC = 47%} {87-97773} {Product size range 251-300...} {reverse primer 408->389} TGGTTGCTTGTAATTCACCG {Tm = 59.6} {PCR product length: 280, GC = 41%} {88-97775} {Product size range 151-200...} {reverse primer 224->205} GCTTCATGAGGAGGAGATGC {Tm = 59.9} {PCR product length: 167, GC = 42%} {89-97784} {Product size range 201-250...} {reverse primer 295->274} GATCCAGTTCTCTCCTTCAACG {Tm = 60.2} {PCR product length: 209, GC = 48%} {90-97785} {Product size range 251-300...} {reverse primer 353->335} ACCAAACCCTTGGTGAAGC {Tm = 60.0} {PCR product length: 251, GC = 49%} {91-97787} {Product size range 151-200...} {reverse primer 342->325} GAGCCCACCAAGCAACAC {Tm = 60.3} {PCR product length: 154, GC = 49%} {92-97792} {Product size range 201-250...} {reverse primer 280->261} TTGATCAGCTACATGCTCCG {Tm = 60.0} {PCR product length: 227, GC = 43%} {93-97793} {Product size range 100-150...} {reverse primer 344->325} CCAATAATCCCTTCGCTTCA {Tm = 60.0} {PCR product length: 105, GC = 49%} {94-97796} {Product size range 151-200...} {reverse primer 237->218} GCTGAAGCGTTCTTCAAGCT {Tm = 59.9} {PCR product length: 171, GC = 45%} {95-97799} {Product size range 151-200...} {reverse primer 284->267} GGAGTGCCGATACCGATG {Tm = 60.0} {PCR product length: 160, GC = 45%} {96-97802} {Product size range 201-250...} {reverse primer 260->242} CCCACCTGTTCCGGATAAG {Tm = 60.3} {PCR product length: 207, GC = 49%}