Arabidopsis EST Mapping Project Plates

Arabidopsis Expressed Sequence Tag (EST) Mapping Project

PLATE 1

Plate 1 (20mers)
A-1
34030 INTERLEUKIN 4 RECEPTO 58
34023 DNAK PROTEIN 72
33865 DINT GENE 360
34041 UBIQUITIN-CONJUGATING ENZYME 424
34169 ?-ORF 57
34110 @? CELLULAS E5 61
34183 TURGOR-REPONSIVE PT 26G 452
33989 KINASE 113
33993 L-POLYGALACTURNONASE INHIBITOR 110\
33896 HYPOTHETICAL 16.5 K PROTEIN 130
34253 POLYLA) BINDING PROTEIN 81
34399
33936 BCD PROTEIN 55
35005 NEUROFILAMENT TRIPLET M PROTEI 62
33876 YL CARRIER PROTEIN 202
33895 NONSPECIFIC LIPID TRANSFER PRO 229
34195 THAUMATIN-LIKE PROTEIN 270
34144 2-OXOIXOVALERATE DEHYDROGENASE 423
34200 SECRECTION PROTEIN XCPT 62
33903 OTEIN KINASE (AT) ill
33893 PHOSPHOGLUCONATE DH 67
33997 RYZAIN BETA CHAIN 526
35189 60S RIBOSOMAL PROTEIN L5A. >pi 324
34418 LZ GLUTATHIONE S-TRANSFERASE 394
33930 ?CARBONYL REDUCTASE 65
33995 ARENICAL PUMP DRIVING ATPASE 120
33911 TOBRB7-18C PROTEIN 548
34144 2-OXOIXOVALERATE DEHYDROGENASE 423
33929
34031 Hypothetical protein - Garden 141
34085 HYPOTHETICAL PROTEIN 57
34512 GLYCINE RICH PROTEIN RNA BINDI 291
33985 PROTEOSOME COMPONENT 187
33916 PROTEASE INHIBITOR P322 202
34263 GLYCE4ALDERYDE 3 @HOSPHATE DH 628
35072 HYPOTHETICAL 20.0 KD PROTEIN I 101
33890 ASPARTIC ACID RICH PROTEIN 65
33815
33840 CYTOSOL AMINOPEPTIDASE (AT) 160
34216 ATHB-2 DNA BINDING PROTEIN 504
33805 @G3P DEHYDROGENASE 494
34010 Zlutamate--ammonia ligase - Ar 661
34315 DNA POLYMERASE 52
34206 HYPOTHETICAL PROTEIN 341
33836 TRANSKETOLASE 197
33859 L-TONOPLAST INTRINSIC PROTEIN GA 541
34473 S-ADENOSYLMETHIONE DECARBOXYLA 173
33812
33901 L-FUCOSE PERMEASE 64
34080 PYRUVATE DECARBOXYLASE 229
34122 IBISCO SMALL CHAIN 3B 491
34009 MYOSIN II 59
34062 PROTEIN G3 61
33983
34021 Inositol-1,4,5-trisphosphate 5 125
34222 GLUCOSE TRANSPORTER PROTEIN 60
34490 PROTEIN KINAS@E C INHIBITOR HOM 326
34032 Tomato MRNA for pectin esteras 53
34268 MYOSIN 72
34359 PHOSPHOGLYCERATE KINASE, CHLOR 211
33870 AUTOANTIGEN SNRNP SM-D 58
33852
34254 V-HEAT SHOCK 70 KD PROTEIN 65
33833 L-DIAMINOPIMELATE EPIMERASE 180
33886 CRUCIFERIN VI 254
33990 CASEIN KINASE II, ALPHA (CKII) 511
33841 HUMAN SCAR PROTEIN,/ 234
34087 ?PPLZO2 PROTEIN 68
34208 GLUTAMATE DECARBOXYLASE 221
34091 40S RIBOSOMAL PROTEIN S8 314
34133 OUTER MEMBRANE COBALAMIN T 62
34255 ALPHA ENOLASE 58
33904 GF14 (AT) PROTEIN KINASE 408
34100 CAB-37 553
33889 LARGE TEGUMENT PROTEIN 59
33843 LARGE T-ANTIGEN 58
34079 HYPOTHETICAL PROTEIN R08D7 223
33855 GLUTAMIC ACID RICH PROTEIN 219
34047 HELICASE 85
33941 MISTONE H2B.2 215
34131 ANNEXIN 225
34556 RIBONUCLEOPROTEIN 74
34312 ORF 63
34783 PROTEASOME COMPONENT C3 (MACRO 227
33954 DNA BINDING PROTEIN PRDII-BF 60
33809 MUCIN 77
33913
33803
33872 EXTENSIN 73
34231 MURINE LEUKEMIA VIRUS 66
34022 GLUTAMINE-AMMONIA LIGASE 469
33976 MYB PROTEIN 308 471
34323 METHIONINE RICH STORAGE PROT 56
34228 LACTOYL GLUTATHIONE LYASE 85
34356 MULTIDRUG RESISTANCE 59
34242

PLATE 4

plate 4 (20mers)

34541 UBIQUiTIN 443
34393
34241 MOBILITY GROUP PROTEIN 63
34371
34492 ELIP STEM II PSII 101
34369
34061 SUPEROXIDE DISMUTASE 562
34514
34234 PYRIMIDINE BINDING PROTEIN 1 88
34149 METHYLAMINE OXIDASE 291
34558 ?GUANINE NUCLEOTIDE DISSOCIAT --59
34147 CINNAMYL-ALCOHOL DEHYDROGENASE 395\
34569 CYTOCHROME P-450 LXXIAL 240
34116 GLUTAMINE SYNTHETASE 446
34158 THYROTROPH EMBRYONIC FACTO 77
34426 L-UBIQUITIN PRECURSOR 446
34057
34564 NADH DH SUBUNIT 6 65
34506 PROTEIN KINASE C INHIBITOR HOM 200
35114 Arabidopsis thaliana tufa gene 544
34602 MITOCHONDRIAL BROWN FAT UNCOUP 88
34175 CHLOROPLAST PHOSPHATE TRANSLOC 448
34083 HYPOTHETICAL (PIR::Sll85O) 163
34153 MUCIN 65
34432 CYCLIC NUCLEOTIDE PHOSPHODIE 58
34209 MOESIN-EZRIN-RADIXIN 63
34348 UL73 GLYCOPROTEIN 53
34491
34823 pal protein (Antirrhinum majus 40
34272 CHL1 GENE PRODUCT 152
34433 3-KETOACYL-COA THIOLASE PEROXI 93
34071 SOLUBLE HYDROGENASE 42 KD SUB 99
34778 KERATIN, TYPE II CYTOSKELETAL 177
34244 SEED LECTIN 106
34571 PG PROTEIN 77
34180 TONOPLAST INTRINSIC GAMMA 467
34203 CHALCONE SYNTHASE 662
34251 TCR-ALPHA GENE 49
34355 NADH DEHYDORGENASE CHAIN PSST 448
34052
34590
34168
34666
34512
34929 cobalamin-independent methioni 263
34436 PHOSPHOGLYCERASTE MUTASE 495
34092
34612 14-3-3-LIKE PROTEIN. >spIP2930 316
34262 ?-MATRIX PROTEIN M2 65
34486 NODULIN-26 HOMOLOG 508
34491
34825 guanylate binding protein (Mus 58
34421
34130 PHOSPHOENOLPYRUVATE CARBOXYLAS 520
34189 ELONGATION FACTOR TU 468
34656 Homo sapienb 59 protein MRNA, 67
34073
34625 LEGUMIN TYPE B PRECURSOR. >gpi 90
34575 63
34181 ORF 58
34305 PROTEIN F3 63
34609 t-Flavonol 4-sulfotransferase- F 137
34043 NADH-UBIQUINONE OXIDOREDUCTASE 56
34479 ELONGATION FACTOR 1 ALPHA-4A 468
34105 NADPH DEHYDORGENASE 133
34429 GLYCINE RICH PROTEIN 449
34232 ANKYRIN-2 71
34881 Mouse MHC H-2K/t-w5-linked ope 86
34540 BACTERIOCHLOROPHYLL SYNTHASE 85
35063 neurofilament protein H form H 107
34101 EBNA-2 NUCLEAR PROTEIN 67\
34188
34567 HISTIDINE RICH GLYCOPROTEIN 214
34462 ?-TRYPSIN INHIBITOR Al 60
34712 eukaryotic protein synthesis i 496
34405 CARBOXYPEPTIDASE Y-LIKE PROTEI 674
34340
34589 t-MALATE DEHYDROGENASE (NADP) 293
34280 POLYCOMB PROTEIN 66
34102 HYPOTHETICAL PROT (PIR::Sl6273 585
34679 GUANINE NUCLEOTIDE-BINDING PRO 285
34079
34577 HYPOTHETICAL PROTEIN 160
34196
34515 CYSTEINE PROTEINASE PRECURSOR 280
34039 transmembrane channel protein 509
34608 PNAJ PROTEIN. >gpIM745691CLOGR 129,
34154 MISTONE H3.3 LIKE 514
34259 CELL SURFACE ANTIGEN 114/AlO g@ 74
34711
34427 KDEL RECEPTOR 221
34245 AUX2 PROTEIN 160
34669
34545 HYPOTHETICAL PROTEIN 58
34118
34207

PLATE 5

{PLATE 5 20-MER}

{1-48917 MYRISTOYL-COA:N-MYRISTOYLTRANSFERASE HUMAN Score: 236}
{2-48938 transforming protein (myb) Arabidopsis Score: 214}
{3-48935 LACTASE-PHLORIZIN HYDROLASE PRECURSOR HUMAN Score: 163}
{4-48946 Maize mitochondrial F-1-ATPase subunit-2 Score: 398}
{5-48956 cytochrome c reductase-processing peptidase subunit I,
MPP, P55 potato Score: 111}
{6-48985 APRT adenine phosphoribosyltransferase Arabidopsis
Score: 390}
{7-49005 bacteriochlorophyll synthase 43K chain Rhodobacter
capsulatus Score: 80}
{8-49036 ribosomal protein L37 - rat Score: 328}
{9-49028 malate dehydrogenase (oxaloacetate-decarboxylating)
poplar score: 311}
{10-49025 H+-transporting ATPase 57K chain Arabidopsis
nucleotide-binding Score: 219}
{11-49022 RNA-binding protein RNP-T Arabidopsis chloroplast
Score: 90}
{12-49043 myb homolog Arabidopsis Score: 209}
{13-49053 proliferating-cell nucleolar antigen P120 human score:
157}
{14-49074 SHIKIMATE 5-DEHYDROGENASE, SHIKIMATE KINASE EPSP SYN
Score: 78}
{15-49088 cysteine proteinase black gram Vigna mungo score: 263}
{16-49082 phosphopyruvate hydratase Arabidopsis score: 293}
{17-49102 heat shock protein 90 [Catharanthus roseus] Score: 398}
{18-49109 phosphomannomutase [Candida albicans] Score: 152}
{19-49120 MENKE DISEASE-ASSOCIATED Cu++-transporting ATPase human
Score: 79}
{20-49175 GDP-D-mannose dehydratase [Escherichia coli] Score:
271}
{21-49168 protein kinase [Lycopersicon pimpinellifolium] Score:
133}
{22-49161 Ran protein/TC4 protein [Lycopersicon esculentum]
score: 393}
{23-49156 DBI gene product [Homo sapiens] Score: 203}
{24-49157 receptor-like protein kinase precursor - Arabidopsis
Score: 168}
{25-49141 ribosomal protein L35a - Arabidopsis Score: 258}
{26-49174 predicted polypeptide for cytokinin-repressed mRNA
[Cucumis sativus] Score: 85}
{27-49196 otsP [Mycobacterium leprae] Score: 107}
{28-49189 ribosomal protein YL30 - human Score: 191}
{29-48657 PUTATIVE ATP-DEPENDENT RNA HELICASE C. elegans Score:
75}
{30-49212 mitochondrial formate dehydrogenase precursor Solanum
tuberosum Score: 306}
{31-49207 transcription factor IID - Arabidopsis Score: 565}
{32-48675 Mouse sex-limited protein (SlpA) gene Score: 202}
{33-48687 cysteine synthase precursor - wheat Score: 240}
{34-48711 superoxide dismutase (Cu-Zn), chloroplast petunia}
{35-48696 CDC2a protein - Arabidopsis p34 protein kinase Score:
310}
{36-48739 cruciferin type C Arabidopsis Score: 287}
{37-48730 phytochrome E [Arabidopsis thaliana] Score: 378}
{38-48772 chorismate mutase Erwinia herbicola score: 128}
{39-48784 initiation factor eIF-5A - common tobacco score: 532 }
{40-48800 X16 protein - mouse pre-mRNA splicing factor [Homo
sapiens] score: 187}
{41-48816 protein kinase - Arabidopsis thaliana Score: 96}
{42-48879 bride of sevenless gene product Drosophila Score: 79}
{43-48870 epsilon-COP [Bos taurus] Score: 231}
{44-48825 S receptor kinase - wild cabbage Score: 94}
{45-48901 Blue copper-binding protein Arabidopsis Score: 423}
{46-48898 inorganic pyrophosphatase, H+-translocating
pyrophosphate-en Score: 381 }
{47-48281 din1 gene product [Raphanus sativus] Score: 146}
{48-48272 cytochrome P450 ALK4, alkane-inducible - yeast Score:
118}
{49-48270 lactoylglutathione lyase human Score: 115 }
{50-48322 Zea mays guanine nucleotide-binding protein beta
subunit Score: 198}
{51-48313 signal recognition particle 54 kDa subunit Arabidopsis
Score: 490}
{52-48297 PCNA [Brassica napus] Score: 256
{53-48288 H.sapiens p63 mRNA for transmembrane protein Score:
146}
{54-48332 ubiquitin/ribosomal polyprotein [Oryza sativa] Score:
357}
{55-48334 phorbol ester-binding protein unc-13 - Caenorhabditis
elegans Score: 106}
{56-48400 fatty-acid desaturase [Anabaena variabilis] Score: 276}
{57-48389 DNA-binding protein petunia Score: 90}
{58-48383 acyl-CoA oxidase I chain A, peroxisomal - rat Score:
278}
{59-48377 hydroxymethylglutaryl-CoA reductase Arabidopsis Score:
511}
{60-48372 betaine-aldehyde dehydrogenase spinach Score: 400}
{61-48368 cytochrome c - cauliflower Score: 401}
{62-48363 major latex protein [Papaver somniferum] Score: 105}
{63-48416 GTP-binding protein rab pea score: 339}
{64-48415 PRT1 protein - yeast Score: 75}
{65-48408 histone H4 - garden pea Score: 375}
{66-48406 growth response protein [Rattus rattus] Score: 126}
{67-48448 GOS2 gene product [Oryza sativa] Score: 120}
{68-48426 photosystem I chain XI - spinach Score: 328}
{69-48479 regulatory protein HAP2 - yeast Score: 174}
{70-48463 T-protein [Solanum tuberosum] Score: 370}
{71-48477 famA protein - Streptomyces purpurascens FAS domain
Score: 107}
{72-48497 pathogenesis-related protein 5 precursor - Arabidopsis
thaumatin-like score: 400}
{73-48503 myo-inositol O-methyl transferase score: 108 }
{74-48526 pollen-specific protein precursor tobacco Score: 141}
{75-48556 glucose-regulated protein 78 Lycopersicon esculentum
Score: 248}
{76-48555 long-chain-fatty-acid--CoA ligase rat Score: 230}
{77-48551 myrosinase - rape Score: 368 }

{78-48534 protein kinase 6 - soybean score: 216}
{79-48530 UTP-glucose glucosyltransferase [Manihot esculenta]
Score: 163}
{80-48574 allene oxide synthase [Linum usitatissimum] Score: 171}
{81-48572 aspartic protease [Brassica oleracea] Score: 279}
{82-48569 N-carbamyl-L-amino acid amidohydrolase Score: 120}
{83-48638 L-ascorbate peroxidase precursor Arabidopsis Score:
514}
{84-48649 ferredoxin-dependent glutamate synthase Spinacia
oleracea Score: 459}
{85-46616 asparagine--tRNA ligase E coli Score: 297}
{86-48653 3-ketoacyl-CoA thiolase [Cucumis sativus] Score: 200}
{87-46619 S.cerevisiae chromosome XI reading frame ORF YKR031c
score: 81}
{88-46628 aldehyde dehydrogenase (NAD+) microsomal - rat Score:
324}
{89-46638 short-chain alcohol dehydrogenase [Picea abies] Score:
181}
{90-46639 INDOLE-3-ACETIC ACID INDUCED PROTEIN ARG7 Vigna radiata
Score: 152}
{91-46707 PROBABLE METABOLITE TRANSPORT PROTEIN Score: 75}
{92-46705 bETA-ADAPTIN CLATHRIN ASSEMBLY PROTEIN COMPLEX 2 Score:
124}
{93-46698 S-phase-specific protein [Brassica rapa] Score: 306}
{94-46699 Protein kinase receptor-like Arabidopsis thaliana
Score: 226}
{95-46674 cadmium efflux adenosine triphosphatase Staphylococcus
aureus Score: 137}
{96-46823 protein kinase, calcium-dependent carrot Score: 84}

PLATE 6

{PLATE 6 20-MER}

{1-46812 gene for homeodomain protein. Score: 146}
{2-46806 DNA topoisomerase - mouse Score: 76}
{3-46804 DNA-binding protein - Arabidopsis Score: 276}
{4-46795 intermediate filament protein [Onchocerca volvulus]
Score: 105}
{5-46759 D.discoideum rasG gene Score: 162}
{6-46747 human cystathionine Score: 120}
{7-46854 GDP dissociation inhibitor - fruit fly Score: 255}
{8-46855 fumarase [Rhizopus oryzae] Score: 288 }
{9-46893 Arginine decarboxylase Tomato Score: 185}
{10-46868 NGR1 protein - yeast Negative growth regulatory protein
Score: 129}
{11-46948 ankyrin B, 440K - human Score: 165}
{12-46943 phosphocarrier protein precursor, mitochondrial -
bovine Score: 87}
{13-46939 deoxycytidylate deaminase Homo sapiens Score: 95}
{14-46935 threonine--tRNA ligase human Score: 241}
{15-46974 aspartate--tRNA ligase rat Score: 272}
{16-46965 Hin-1 protein - human Score: 82}
{17-47092 tobacco (N.plumbaginifolia) beta-glucanase mRNA, 3'
end. Score: 199}
{18-46995 epoxide hydrolase Solanum tuberosum Score: 196 }
{19-47144 multidrug resistance protein 1 - Entamoeba histolytica
Score: 197 }
{20-47174 protoporphyrin IX magnesium chelatase snapdragon Score:
415 }
{21-47233 UTP glucose glucosyltransferase Manihot esculenta.
Score: 2075}
{22-47234 FVT1 protein - human Score: 100}
{23-47199 UDP rhamnose-anthocyanidin-3-glucoside
rhamnosyltransferase Score: 100 }
{24-47197 MOUSE T-COMPLEX PROTEIN 1, ZETA SUBUNIT Score: 138}
{25-47192 probable methyltransferase ERG6 - yeast Score: 106 }
{26-47243 cytosolic triose phosphate isomerase [Arabidopsis
thaliana] Score: 120}
{27-47244 ARATH CHLORATE/NITRATE TRANSPORTER. CHL1 Arabidopsis
Score: 121}
{28-47245 3-isopropylmalate dehydrogenase rape Score: 211}
{29-47239 hydroxymethylbilane synthase Arabidopsis Score: 319 }
{30-49231 development-specific protein 217 sea urchin Score: 146
}
{31-49244 succinic semialdehyde dehydrogenase E coli Score: 95}
{32-49243 plasma membrane intrinsic protein 1c [Arabidopsis
thaliana] Score: 383}
{33-49299 calmodulin - Plasmodium falciparum Score: 138 }
{34-49295 troponin C, fast skeletal muscle human Score: 143}
{35-49287 precursor (clone PNC2) cationic peroxidase Arachis
hypogaea Score: 259 }
{36-49279 H(+)-transporting ATPase sub E Manduca sexta Score: 82}
{37-49278 cysteine proteinase inhibitor cowpea Score: 282 }
{38-49274 receptor protein kinase TMK1 Arabidopsis Score: 509 }
{40-49272 chorismate mutase Erwinia herbicola Score: 128 }
{41-49269 peroxisomal membrane protein 20K B - yeast Score: 92}
{42-49268 Athb-6 gene product [Arabidopsis thaliana] Score: 369}
{43-49253 triose-phosphate isomerase (Schistosoma mansoni) Score:
80}
{44-49246 ALPHA-GALACTOSIDASE PRECURSOR Cyamopsis tetragonoloba
Score: 125 }
{45-49350 iron-responsive element-binding protein mouse Score:
100}
{46-49345 hypothetical protein turnip S-phase-specific protein
Score: 324 }
{47-49330 phorbol ester-binding protein unc-13 - C. elegans
Score: 90}
{48-49324 protein kinase=pll Drosophila pelle protein Score: 113}
{49-49356 metallothionein-like protein [Actinidia deliciosa]
Score: 93}
{50-49355 regulatory protein G1 fruit fly goliath Score: 87}
{51-49405 trehalose-phosphatase yeast Score: 154}
{52-49398 SUGAR-NUCLEOTIDYLATION ENZYME Streptomyces griseus
Score: 141}
{53-49395 thioglucosidase [Arabidopsis thaliana] Score: 567}
{54-49386 cationic peroxidase 2 precusor [Arabidopsis thaliana]
Score: 264}
{55-49383 formate dehydrogenase potato mitochondrion Score: 258}
{56-49356 metallothionein-like protein [Actinidia deliciosa]
Score: 93}
{57-49455 ELI3-2 protein Arabidopsis Score: 435 }
{58-49483 strictosidine synthase [Catharanthus roseus] Score:
114}
{59-49480 Drosophila virilis mastermind gene Score: 265}
{60-49502 Spinach mRNA for photosystem I subunit V [Spinacia
oleracea] Score: 312 }
{61-49486 F42G9.1 gene product [Caenorhabditis elegans] Score: 80
}
{62-49517 tryptophan 2-monooxygenase Pseudomonas syringae pv.
savastanoi score: 77}
{63-49512 glycine-rich protein (clone AtGRP8) Arabidopsis
thaliana Score: 418}
{64-49504 NADH dehydrogenase (ubiquinone) chain CI-B14 - bovine
score: 112}
{65-49582 granaticin-producing polyketide synthase chain 5
Streptomyces Score: 152}
{66-49575 initiation factor eIF-4A1 Arabidopsis translation
initiation score: 613}
{67-49535 p63 transmembrane protein human Score: 162}
{68-49528 RNA-binding protein Arabidopsis glycine rich protein
score: 426}
{69-49522 metallothionein I Arabidopsis Score: 182 }
{70-49581 psaE subunit III of photosystem I reaction centre
Spinacia oleracea Score: 213}
{71-49587 aspartate transaminase narrow-leaved blue lupine Score:
413}
{72-49589 zinc-finger protein [Petunia hybrida] Score: 87}
{73-49596 multicatalytic endopeptidase complex chain C8 rat
Score: 294}
{74-49640 abscisic acid and stress inducible (A22) Hordeum
vulgare Score: 233}
{75-49626 P.sativum legK gene (clone lambda LegJ2) Score: 154 }
{76-49604 6-phosphogluconate dehydrogenase [Synechococcus sp.]
Score: 266 }
{77-49693 Dictyostelium discoiedeum AAC-rich mRNA (AAC3) Score:
202}
{78-49685 UDPglucose 4-epimerase S. thermophilus Score: 78 }
{79-49681 acyl-CoA oxidase [Homo sapiens] Score: 222}
{80-49676 ATP sulfurylase [Arabidopsis thaliana] Score: 465}
{81-49663 PSI-H precursor [Nicotiana sylvestris] Score: 216}
{82-49645 photosystem II 10K protein Arabidopsis Score: 369}
{83-49642 ATP-dependent Clp proteinase chain P E coli Score: 105}
{84-52026 ATTS3331}
{85-52031 ATTS3332}
{86-52032 glutamate decarboxylase Petunia hybrida Score: 528}
{87-5203 3-deoxy-D-arabino-heptulosonate 7-phosphate synthasE
Score: 4639}
{88-52052 ATTS3299}
{89-52054 ATTS3295}
{90-52059 FAFJ86; 3' end; Overlap with FAFJ86-2}
{91-52062 chaperonin groES homolog Arabidopsis thaliana Score:
250}
{92-52081 cationic peroxidase [Arachis hypogaea] Score: 265}
{93-52087 ATTS3307}
{94-52093 ATTS3333}
{95-52094 RAS-related GTP-binding protein Pisum sativum Score:
390}
{96-52099 inorganic pyrophosphatase Ara Score: 183}