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SIMPLE
SPECTROPHOTOMETRIC DETERMINATION OF TRACES OF SELENIUM IN ENVIRONMENTAL
SAMPLES
Krishnaiah,
L., K. Suresh Kumar, K. Suvardhan, P. Chiranjeevi “Simple
Spectrophotometric Determination Of Traces Of Selenium In Environmental
Samples” in Martin J. Bunch, V. Madha Suresh and T. Vasantha
Kumaran, eds., Proceedings of the Third International Conference
on Environment and Health, Chennai, India, 15-17 December, 2003.
Chennai: Department of Geography, University of Madras and Faculty
of Environmental Studies, York University. Pages 217 – 225.
Abstract:
A rapid, highly sensitive
and selective spectrophotometric method for the determination
of traces of selenium(IV) are studied. The methods are based on
either the oxidation of 2,4-dinitrophenyl hydrazine hydrochloride
(2,4-DNPH) by selenium in hydrochloric acid medium and coupling
with N-(1-naphthyl) ethylenediamine dihydrochloride(NEDA) to give
pink colored product or the oxidation of 4-aminoresorcinol hydrochloride
(4-ARCH) by selenium in sulphuric acid medium and coupling with
4-aminoresorcinol hydrochloried to yield a orange red colored
product. The pink colored product with lmax 520 nm is stable for
24 h and the orange red colored product with l max 495 nm for
one week. Beer’s law is obeyed for selenium in the concentration
range 0.03 - 3.5 µg ml-1 (pink color product) and 0.07-2.5
µg ml-1 (orange red color product), respectively. The optimum
reaction conditions and other important analytical parameters
are reported. Interference due to various cations and anions have
been investigated. The proposed method has been applied to the
analysis of polluted water, natural water, plant material, soil
samples and synthetic mixture. The results of analysis are compared
with the reported method.
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