ICEH Papers

PRECONCENTRATION OF LEAD USING MERCAPTOSEPHADEX AND ITS DETERMINATION WITH NEW REAGENT BY PECTROPHOTOMETRY

Kumar, K. Suresh, K. Suvardhan, B. Lokanath Swaroop, P. Chiranjeevi “Preconcentration Of Lead Using Mercaptosephadex And Its Determination With New Reagent By Spectrophotometry” in Martin J. Bunch, V. Madha Suresh and T. Vasantha Kumaran, eds., Proceedings of the Third International Conference on Environment and Health, Chennai, India, 15-17 December, 2003. Chennai: Department of Geography, University of Madras and Faculty of Environmental Studies, York University. Pages 252 – 262.

Abstract:

A highly sensitive and selective spectrophotometric method for the determination of traces of Lead in water after pre-concentration using Mercapto sephadex (MS-50) has been developed. The method is based on the color reaction of lead (II) with dichlorohydroxyl phenyl porphyrin. Under the optimum condition, Pb(II) reacts with reagent to from 1:2 yellow complex in presence of Triton X-100, which has a maximum absorption peak at 482 nm. The color reaction can be completed rapidly and remain stable for more than 24 h at room temperature. The molar absorption co-efficient of the lead complex, the limit of quantification, the limit of detection and relative standard deviations were found to be 2.45 H 105 L Mol-1 cm-1, 4.4, 1.45 ng ml-1 and 1% respectively. The absorbance of the lead complex at 482 nm is linear up to 0.46 µg ml-1 of lead(II). The effect of various co-existing ions in water were examined seriously. No interfere was observed. Moreover, a simple pre-concentration method for trace of lead in water was also studied using MS-50. It was found that traces in lead in water can be absorbed.

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