ICEH Papers

SPECTROFLUOROMETRIC DETERMINATION OF DIMETHOATE RESIDUES IN ENVIRONMENTAL SAMPLES

Swaroop, A. Lokanath, S. Prabhakar Rao, L. Krishnaiah, P. Chiranjeevi “Spectrofluorometric Determination Of Dimethoate Residues In Environmental Samples” in Martin J. Bunch, V. Madha Suresh and T. Vasantha Kumaran, eds., Proceedings of the Third International Conference on Environment and Health, Chennai, India, 15-17 December, 2003. Chennai: Department of Geography, University of Madras and Faculty of Environmental Studies, York University. Pages 570 -576.

Abstract:

A simple, sensitive and extractive spectrofluorimetric method for the determination of dimethoate is described. Dimethoate was hydrolysed with sodium-ethoxide in to sodium dimethyl dithiophosphate (Na-DMDTP); the Na-DMDTP was extracted with cationic fluorescein dye as an ion pair in to 1-chloro 2-nitrobenzene which greatly increases the sensitivity. The fluorescence of the organic layer was measured at 528 nm after exitation at 485 nm under the optimal conditions. Beer’s law is obeyed over the range from 5.0 mg to 60.0 mg of DMDTP. The limit of detection [LOD], variation co-efficient, the limit of quantification [LOQ] where 5.0 mg mL-1, 1.0% and 60.0 mg mL-1 respectively. The proposed method has been applied successfully for the determination of dimethoate residues in water samples up to the ppb level with preconcentration on amberlite XAD-4.

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