Root Hairs. An
Arabidopsis thaliana root hair impaled and injected with the fluorescent dye lucifer yellow. Note the cytoplasmic location of the dye,
hence the cytoplasmic location of the micropipette tip (the lucifer yellow dye preferentially accumulates in the
nuclei). The micropipette is used to measure the electrical properties of the root hair.
Image: Continued growth of a root hair after impalement (and removal of the micropipette). Time intervals are 2 or 3 minutes.
Publications
- Lew RR (2018) Nano-engineering pollination (commentary). New Phytologist. 220:8-9. doi:10.1111/nph.15358. [pubmed]
- Lew RR (2009) Root hair electrophysiology. In Emons, AMC and T Ketelaar (eds.) Root Hairs. Plant Cell Monographs Volume 12. Springer-Verlag. Berlin, Heidelberg (invited review) pp. 123-144. [link via DOI] Submitted Chapter [pdf]
- Lew RR (2004) Osmotic effects on the
electrical properties of Arabidopsis thaliana L. root hair vacuoles
in situ. Plant Physiol. 134:352-360.
[pubmed]
electrical circuitry for measuring plasma and vacuolar membrane electrical properties [.png format]
- Shabala S and RR Lew (2002) Turgor regulation in osmotically stressed Arabidopsis thaliana epidermal root cells: Direct support for the role of inorganic ion uptake as revealed by concurrent flux and cell turgor measurements. Plant Physiology. 129:290-299. [pubmed]
- Babourina O, B Hawkins, RR Lew, I Newman and S Shabala (2001) K+ transport by Arabidopsis root hairs at low pH. Australian Journal of Plant Physiology. 28:637-643. [abstract]
- Lew RR (2000) Electrobiology of root hairs. In Ridge RW and AM Emons (eds.) Cell and Molecular Biology of Root Hairs. Springer Verlag. Tokyo (invited review) pp. 115-139. [link via DOI]
- Lew RR, JDW Dearnaley (2000) Nucleotide effects on the electrical properties of growing Arabidopsis thaliana root hairs. Plant Science. 153:1-6. [abstract]
- Covic L, NF Silva, RR Lew (1999) Functional characterization of ARAKIN (ATMEKK1): a possible mediator in an osmotic stress response pathway in higher plants. Biochimica Biophysica Acta. 1451:242-254. [pubmed]
- Lew RR (1998) Immediate and steady state extracellular ionic fluxes of growing Arabidopsis thaliana root hairs under hyperosmotic and hypoosmotic conditions. Physiologia Plantarum. 10 4:397-404. [abstract]
- Lew RR (1996) Pressure regulation of the electrical properties of growing
Arabidopsis thaliana L. roothairs. Plant Physiology.
112:1089-1100.
[pubmed]
Pressure and electrical coupling movie [8.6 MB .mov format] [2.9 MB .mp4 format]. Pressure probe and double barrel micropipette impalement of a root hair [3.5 MB .mov format] [0.8 MB .mp4 format], followed by silicone oil injection into the root hair [15.4 MB .mov format] [1.4 MB .mp4 format].
![[.png format]](images/root_hair_circuit.png){kind=link}
- Covic L and RR Lew (1996) Arabidopsis thaliana cDNA isolated by functional complementation shows homology to serine/threonine kinases. Biochimica Biophysica Acta. 1305:125-129. [pubmed]
- Lew RR (1994) Regulation of electrical coupling between Arabidopsis root hairs. Planta. 193:67-73. [abstract]
- Lew RR (1991) Electrogenic transport properties of growing Arabidopsis root hairs: the plasma membrane proton pump and potassium channels. Plant Physiology. 97:1527-1534. [pubmed]
- Lew RR (1991) Substrate regulation of potassium and chloride channels in Arabidopsis plasma membrane. Plant Physiology. 95:642-647. [pubmed]
Images: Triple-barrel micropipette (upper) and seedling growth on gellan gum substrate (lower).