Proteins adopt a number of different conformations and modified states that occur both in the cell and during biopharmaceutical manufacturing. Identifying and quantifying these proteoforms is critical for developing a fundamental understanding of their function in health and disease, as well as providing information on their safety, efficacy, and stability as protein-based biotherapeutics.
Proteomics is the qualitative and quantitative study of the proteome. With our TimsToF Pro 2 and Nanoelute 2 system, the facility can support different proteomic workflows such as:
- iTRAQ and Tandem Mass Tag (TMT) Labelling.
- Label Free Quantification.
- Protein identification.
- Peptide mapping.
The TimsToF Pro 2 is uniquely suited with its ion mobility technology for such purpose and can provide multi-attribute methods (MAM) to monitor critical quality attributes including Post-Translational Modifications like Deamidation, Phosphorylation, Methylation, Oxidation, Glycosylation, among others.
