Laboratory Equipment

 

  • Ryan Hili

    Mermade 12 DNA Synthesizer

    12-port parallel DNA synthesizer

    Can rapidly generate heavily modified oligos in high yield

    Great for combinatorial library development

  • Agilent 1260 HPLC

    Analytical and Semi-preparative scales

    Auto-sampler and fraction-collector

  • Nanodrop Spectrophotometer

    Rapidly quantify protein and oligonucleotides

    Uses just 1 uL per experiment

    • Ryan Hili

      Quantitative/Real-Time PCR System

      Qualitative and quantitaive analysis of oligonucleotides

    • Touch-Screen Thermal Cyclers (two)

      Rapid and uniform heating and cooling

      Gradient for optimization

    • Gel Imager

      Quick automatic imaging of gels

      EtBR, SYBR green/gold, Fluorescein, etc...

    • Ryan Hili

      Centrifuge

      24-sample model

      PCF/RPM display, >20,000g, very quiet

    • Lyophilizer

      4.5 L system

      Held at 0.006 mbar/ -93 °C

    • Centrivap

      Custom ordered for our oligo synthesis needs

    • Ryan Hili

      Analytical Balance

      Analytical semi-micro auto-calibrating balance

      0.01 mg readability, better than 0.02 mg repeatability

    • Gel Station

      Typical set-up

      Small oven used for denaturing PAGE

    • Rotovap

      Complete Buchi system

      Digital vaccum control/program

    • Ryan Hili

      Water Purification System

      ELGA PURELAB flex 3/ RO, UV and DI for Type I water

      Real-time monitoring of water purity

    • Fume Hoods (six)

      6 double length fume hoods (3 per lab)

      High-vacuum manifolds

    • Lab Space

      2 labs located in 543 and 543 of the chemistry building

      Moisture control, -20 °C freezers, fridges, etc